Exploring Glycogen Processing Enzymes in Trichomonas vaginalis: Cloning,Characterization and Implications for Carbohydrate Metabolism and CervicalCancer Risk

Patel, Sharma, Bansal, Raju, Chanania and Laddad

Health Leadership and Quality of Life, 2025, vol. 4, 776

Abstract: Trichomonas vaginalis, often known as T. vaginalis, is a protozoan that causes trichomoniasis, a prevalent sexually transmitted disease (STD) and a serious human pathogen. Glycogen is a branching polymer of glucose that the individual has been identified to accumulate for an elongated instance and then use when carbohydrates are insufficient. The crucial enzymes involved in glycogen production and investigate; Trichomonas vaginalis glycogen (TVG) syntheses and glycogen phosphorylase were considered. Research found that their regulatory features were different from known enzymes in animals and fungi. Bacterial recombinant synthesis of the open reading frames (ORF) TVAG 276310 produced proteins exhibiting branch and de-branch activity. Specifically, transgenic TVAG_276310 shows a preference for polysaccharides with long outside branches, which can include a substance and amylose undergrowth. Three proteins encompassed in the glucosidase formulation were identified by fluid chromatography and tandem mass spectrometry. The most notable was a putative-amylase, encoded by the TVAG_080000 ORF. Glycogen increase and branching were standardized after transgenic production of yeast cells devoid of enzyme movement that branches or de-branch glycogen.

Date: 2025
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Persistent link: https://EconPapers.repec.org/RePEc:dbk:health:v:4:y:2025:i::p:776:id:776

DOI: 10.56294/hl2025776

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