Prevalence of Adhesion Genes fnbA & fnbB of Staphylococcus aureus Isolated from Dental Patients Attending Dentistry Department of Tehsil Headquarter Hospital Pasrur, Pakistan

Muhammad Danish Mehmood, Huma Anwar Ul-Haq, Khushbu Farva, Zuhaib Farooq, Gul Muhammad Shaikh, Muhammad Usman Ghani and Verda Naeem
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Muhammad Danish Mehmood: Ottoman Pharma, Pakistan.
Huma Anwar Ul-Haq: Ottoman Pharma, Pakistan.
Khushbu Farva: Shara Medical College, Narowal, Pakistan.
Zuhaib Farooq: Surgident Executive Dental Clinic, Pakistan.
Gul Muhammad Shaikh: Shahida Islam Medical & Dental College, Pakistan.
Muhammad Usman Ghani: Ottoman Pharma, Pakistan.
Verda Naeem: Rawal Medical and Dental College, Pakistan.

European Journal of Dental and Oral Health, 2020, vol. 1, issue 4

Abstract: Introduction: The present study was intended to isolate, characterize and investigate the prevalence of virulence genes encoding fibronectin (fnbA and fnbB) adhesive molecules of S. aureus from dental patients attending outpatient department of THQ Hospital Pasur. Methodology: A total of 100 oral samples were collected from dental patients, pure cultures was segregated and identified through conventional microbiological methods to evaluate the prevalence of S. aureus. Isolates were further characterized by using specific primers for genotype fnbA and fnbB. Results: Results of the study declared that 68 samples were positive (68%) for staphylococcus aureus on the basis of growth on selective media and appearance of typical colonies supported by gram staining. These gram positive staphylococci were positive (86.7%) in coagulase and catalase testing. The results of polymerase chain reaction (PCR) revealed that 47 (69.1%) and 23 (33.8%) isolates showed amplification with type specific primer 23Sr RNA and NUC gene respectively. Furthermore, fnbA and fnbB type specific genes of S. aureus did not show any amplification in PCR reaction. Conclusion: Irrespectively the data in the present study showed the prevalence of S. aureus is significantly high in males and of age group of 20-40 years but no positive result was found for prevalence of fnbA and fnbB genes. All the S. aureus isolates were highly sensitive to vancomycin, linezolid and clindamycin.

Keywords: Staphylococcus aureus; fnbA; fnbB; Polymerase chain reaction (PCR) (search for similar items in EconPapers)
Date: 2020
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Persistent link: https://EconPapers.repec.org/RePEc:epw:ejdent:v:1:y:2020:i:4:id:13006

DOI: 10.24018/ejdent.2020.1.4.6

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