Glucose-Stimulated Kinetic Model for the Selection of β-glucosidase for Industrial Processes

Saarthak Shukla

International Journal of Scientific Research in Science and Technology, 2026, vol. 13, issue 2, 292-297

Abstract: Efficient cellulose hydrolysis for bioethanol production is limited by its end product glucose as it inhibits β-glucosidases, the final enzyme in the multi-enzyme hydrolysis. Therefore, a glucose tolerant β-glucosidase with improved activity at high glucose concentrations is essential to overcome this product inhibition. Although a variety of such β-glucosidases have been identified or engineered, a kinetic model that can compare their performance under industrially relevant conditions is valuable for optimizing enzyme selection. In this study, a glucose-stimulated kinetic model was developed by extending an existing activation model to incorporate glucose inhibition effects. The model response was evaluated using previously reported β-glucosidase hydrolysis data of p-nitrophenyl-β-D-glucopyranoside (pNP-Glu). The resulting kinetic parameters, including glucose inhibition constants and maximum specific activity provide a quantitative framework for selecting suitable β-glucosidases for industrial bioethanol production.

Keywords: Glucose-Stimulated Kinetic Model; Selection Of -glucosidase; -glucosidase For Industrial; Industrial Processes (search for similar items in EconPapers)
Date: 2026
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Persistent link: https://EconPapers.repec.org/RePEc:etm:ijsrst:v13:y2026:i2:id:1452

DOI: 10.32628/IJSRST2613204

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