Comparative Analysis of CAZymes from Trichoderma longibrachiatum LMBC 172 Cultured with Three Different Carbon Sources: Sugarcane Bagasse, Tamarind Seeds, and Hemicellulose Simulation

Alex Graça Contato (), Tiago Cabral Borelli, Ana Karine Furtado de Carvalho, Heitor Buzetti Simões Bento, Marcos Silveira Buckeridge, Janet Rogers, Steven Hartson, Rolf Alexander Prade and Maria de Lourdes Teixeira de Moraes Polizeli
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Alex Graça Contato: Department of Biochemistry and Immunology, Faculty of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto 14040-900, SP, Brazil
Tiago Cabral Borelli: Department of Cellular and Molecular Biology and Pathogenic Bioagents, Faculty of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto 14040-900, SP, Brazil
Ana Karine Furtado de Carvalho: Department of Basic and Environmental Sciences, Lorena School of Engineering, University of São Paulo, Lorena 12602-810, SP, Brazil
Heitor Buzetti Simões Bento: Department of Bioprocess Engineering and Biotechnology, School of Pharmaceutical Sciences, São Paulo State University, Araraquara 14800-903, SP, Brazil
Marcos Silveira Buckeridge: Department of Botany, Institute of Biosciences, University of São Paulo, São Paulo 05508-090, SP, Brazil
Janet Rogers: Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, OK 74078, USA
Steven Hartson: Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, OK 74078, USA
Rolf Alexander Prade: Department of Microbiology and Molecular Genetics, Oklahoma State University, Stillwater, OK 74078, USA
Maria de Lourdes Teixeira de Moraes Polizeli: Department of Biochemistry and Immunology, Faculty of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto 14040-900, SP, Brazil

Clean Technol., 2024, vol. 6, issue 3, 1-17

Abstract: The examination of fungal secretomes has garnered attention for its potential to unveil the repertoire of secreted proteins, notably CAZymes (Carbohydrate-Active enzymes), across various microorganisms. This study presents findings on categorizing the secretome profile of CAZymes by their function and family, derived from the filamentous fungus Trichoderma longibrachiatum LMBC 172. The cultivation was performed through submerged fermentation with three distinct carbon sources: sugarcane bagasse, tamarind seeds, and a control simulating hemicellulose containing 0.5% beechwood xylan plus 0.5% oat spelt xylan. The secretome analysis revealed 206 distinct CAZymes. Each carbon source showed particularities and differences. Of these, 89 proteins were produced simultaneously with all the carbon sources; specifically, 41 proteins using only the hemicellulose simulation, 29 proteins when sugarcane bagasse was used as a carbon source, and only 3 when tamarind seeds were used. However, in this last condition, there was a high intensity of xyloglucanase GH74 production, thus reaffirming the richness of xyloglucan in the constitution of these seeds. When evaluating the proteins found in two conditions, 18 proteins were shown between the simulation of hemicellulose and sugarcane bagasse, 11 proteins between the simulation of hemicellulose and tamarind seeds, and 15 proteins between sugarcane bagasse and tamarind seeds. Among the proteins found, there are representatives of different families such as glycosyl hydrolases (GHs) that cleave cellulose, hemicellulose, pectin, or other components; carbohydrate esterases (CEs); polysaccharide lyases (PLs); carbohydrate-binding modules (CBMs); and auxiliary activity enzymes (AAs). These results demonstrate the importance of analyzing CAZymes secreted by microorganisms under different culture conditions.

Keywords: CAZymes; secretome; Trichoderma longibrachiatum; sugarcane bagasse; tamarind seeds (search for similar items in EconPapers)
JEL-codes: Q2 Q3 Q4 Q5 (search for similar items in EconPapers)
Date: 2024
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