Impact of p53 Knockout on Protein Data Set of HaCaT Cells in Confluent and Subconfluent Conditions

Alexander L. Rusanov, Daniil D. Romashin, Peter M. Kozhin, Maxim N. Karagyaur, Dmitry S. Loginov, Olga V. Tikhonova, Victor G. Zgoda and Nataliya G. Luzgina
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Alexander L. Rusanov: V. N. Orekhovich Research Institute of Biomedical Chemistry, 119121 Moscow, Russia
Daniil D. Romashin: V. N. Orekhovich Research Institute of Biomedical Chemistry, 119121 Moscow, Russia
Peter M. Kozhin: V. N. Orekhovich Research Institute of Biomedical Chemistry, 119121 Moscow, Russia
Maxim N. Karagyaur: V. N. Orekhovich Research Institute of Biomedical Chemistry, 119121 Moscow, Russia
Dmitry S. Loginov: V. N. Orekhovich Research Institute of Biomedical Chemistry, 119121 Moscow, Russia
Olga V. Tikhonova: V. N. Orekhovich Research Institute of Biomedical Chemistry, 119121 Moscow, Russia
Victor G. Zgoda: V. N. Orekhovich Research Institute of Biomedical Chemistry, 119121 Moscow, Russia
Nataliya G. Luzgina: V. N. Orekhovich Research Institute of Biomedical Chemistry, 119121 Moscow, Russia

Data, 2022, vol. 7, issue 3, 1-8

Abstract: The immortalized keratinocytes, HaCaT, are a popular model for skin research (toxicity, irritation, allergic reactions, or interaction of cells). They maintain a stable keratinocyte phenotype and respond to keratinocyte differentiation stimuli. However, programs of stratification and expression of differentiation markers in HaCaT keratinocytes are aberrant. In HaCaT cells, there are two mutant p53 alleles (i.e., R282Q and H179Y) that contain gain-of-function (GOF) mutations resulting from spontaneous immortalization (mutp53). At the same time, mutp53 acts as a transcription factor and also affects the interaction of p63 protein with its transcription targets. Proteins of the p53 family are crucial for regulation of proliferation and differentiation processes in human keratinocytes, although the involvement of mutp53 in these processes is not fully clear. We present data sets obtained as a result of high-performance proteomic analysis of immortalized HaCaT keratinocytes with p53 knockout in two different states, subconfluent and confluent, which are characterized by different intensites of cell differentiation processes. To obtain the proteomic profiles of the cells, we applied LC-MS/MS measurements processed with MaxQuant software (version 1.6.3.4).

Keywords: keratinocyte; p53; proteome; LC-MS/MS; knockout; confluent (search for similar items in EconPapers)
JEL-codes: C8 C80 C81 C82 C83 (search for similar items in EconPapers)
Date: 2022
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