Dataset: Impact of β-Galactosylceramidase Overexpression on the Protein Profile of Braf(V600E) Mutated Melanoma Cells

Davide Capoferri, Paola Chiodelli, Stefano Calza, Marcello Manfredi and Marco Presta ()
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Davide Capoferri: Department of Molecular and Translational Medicine, University of Brescia, 25123 Brescia, Italy
Paola Chiodelli: Department of Molecular and Translational Medicine, University of Brescia, 25123 Brescia, Italy
Stefano Calza: Department of Molecular and Translational Medicine, University of Brescia, 25123 Brescia, Italy
Marcello Manfredi: Department of Translational Medicine, University of Piemonte Orientale, 13100 Novara, Italy
Marco Presta: Department of Molecular and Translational Medicine, University of Brescia, 25123 Brescia, Italy

Data, 2023, vol. 8, issue 12, 1-7

Abstract: β-Galactosylceramidase (GALC) is a lysosomal enzyme involved in sphingolipid metabolism by removing β-galactosyl moieties from β-galactosyl ceramide and β-galactosyl sphingosine. Previous observations have shown that GALC exerts a pro-oncogenic activity in human melanoma. Here, the impact of GALC overexpression on the proteomic landscape of BRAF -mutated A2058 and A375 human melanoma cell lines was investigated by liquid chromatography–tandem mass spectrometry analysis of the cell extracts. The results indicate that GALC overexpression causes the upregulation/downregulation of 172/99 proteins in GALC -transduced cells when compared to control cells. Gene ontology categorization of up/down-regulated proteins indicates that GALC may modulate the protein landscape in BRAF -mutated melanoma cells by affecting various biological processes, including RNA metabolism, cell organelle fate, and intracellular redox status. Overall, these data provide further insights into the pro-oncogenic functions of the sphingolipid metabolizing enzyme GALC in human melanoma.

Keywords: melanoma; proteomics; sphingolipids; ?-galactosylceramidase (search for similar items in EconPapers)
JEL-codes: C8 C80 C81 C82 C83 (search for similar items in EconPapers)
Date: 2023
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