Proteomic Shotgun and Targeted Mass Spectrometric Datasets of Cerebrospinal Fluid (Liquor) Derived from Patients with Vestibular Schwannoma

Svetlana Novikova (), Natalia Soloveva, Tatiana Farafonova, Olga Tikhonova, Vadim Shimansky (), Ivan Kugushev and Victor Zgoda ()
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Svetlana Novikova: Institute of Biomedical Chemistry, 119121 Moscow, Russia
Natalia Soloveva: Institute of Biomedical Chemistry, 119121 Moscow, Russia
Tatiana Farafonova: Institute of Biomedical Chemistry, 119121 Moscow, Russia
Olga Tikhonova: Institute of Biomedical Chemistry, 119121 Moscow, Russia
Vadim Shimansky: N.N. Burdenko National Scientific and Practical Center for Neurosurgery, 125047 Moscow, Russia
Ivan Kugushev: N.N. Burdenko National Scientific and Practical Center for Neurosurgery, 125047 Moscow, Russia
Victor Zgoda: Institute of Biomedical Chemistry, 119121 Moscow, Russia

Data, 2023, vol. 8, issue 4, 1-10

Abstract: Vestibular schwannomas are relatively rare intracranial tumors compared to other brain tumors. Data on the molecular features, especially on schwannoma proteome, are scarce. The 41 cerebrospinal fluid (liquor) samples were obtained during the surgical removal of vestibular schwannoma. Obtained peptide samples were analyzed by shotgun LC-MS/MS high-resolution mass spectrometry. The same peptide samples were spiked with 148 stable isotopically labeled peptide standards (SIS) followed by alkaline fractionation and scheduled multiple reaction monitoring (MRM) for quantitative analysis. The natural counterparts of SIS peptides were mapped onto 111 proteins that were Food and Drug Administration (FDA)-approved for diagnostic use. As a result, 525 proteins were identified by shotgun LC-MS/MS with high confidence (at least two peptides per protein, FDR < 1%) in liquor samples. Absolute quantitative concentrations were obtained for 54 FDA-approved proteins detected in at least five experimental samples. Since there is lack of data on the molecular landscape of vestibular schwannoma, the obtained datasets are unique and one of the first in its field.

Keywords: mass spectrometry; proteomics; shotgun; targeted mass spectrometry; MRM/SIS; vestibular schwannoma (search for similar items in EconPapers)
JEL-codes: C8 C80 C81 C82 C83 (search for similar items in EconPapers)
Date: 2023
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