DNA Methylome and Transcriptome Maps of Primary Colorectal Cancer and Matched Liver Metastasis

Priyadarshana Ajithkumar, Gregory Gimenez, Peter A. Stockwell, Suzan Almomani, Sarah A. Bowden, Anna L. Leichter, Antonio Ahn, Sharon Pattison, Sebastian Schmeier, Frank A. Frizelle, Michael R. Eccles, Rachel V. Purcell (), Euan J. Rodger () and Aniruddha Chatterjee ()
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Priyadarshana Ajithkumar: Department of Pathology, Dunedin School of Medicine, University of Otago, Dunedin 9016, New Zealand
Gregory Gimenez: Department of Pathology, Dunedin School of Medicine, University of Otago, Dunedin 9016, New Zealand
Peter A. Stockwell: Department of Pathology, Dunedin School of Medicine, University of Otago, Dunedin 9016, New Zealand
Suzan Almomani: Department of Pathology, Dunedin School of Medicine, University of Otago, Dunedin 9016, New Zealand
Sarah A. Bowden: Department of Pathology, Dunedin School of Medicine, University of Otago, Dunedin 9016, New Zealand
Anna L. Leichter: Department of Pathology, Dunedin School of Medicine, University of Otago, Dunedin 9016, New Zealand
Antonio Ahn: Peter MacCallum Cancer Centre, Melbourne, VIC 3052, Australia
Sharon Pattison: Department of Medicine, Dunedin School of Medicine, University of Otago, Dunedin 9016, New Zealand
Sebastian Schmeier: Evotec SE, 22419 Hamburg, Germany
Frank A. Frizelle: Department of Surgery, University of Otago, Christchurch 8011, New Zealand
Michael R. Eccles: Department of Pathology, Dunedin School of Medicine, University of Otago, Dunedin 9016, New Zealand
Rachel V. Purcell: Department of Surgery, University of Otago, Christchurch 8011, New Zealand
Euan J. Rodger: Department of Pathology, Dunedin School of Medicine, University of Otago, Dunedin 9016, New Zealand
Aniruddha Chatterjee: Department of Pathology, Dunedin School of Medicine, University of Otago, Dunedin 9016, New Zealand

Data, 2023, vol. 9, issue 1, 1-12

Abstract: Sequencing-based genome-wide DNA methylation, gene expression studies and associated data on paired colorectal cancer (CRC) primary and liver metastasis are very limited. We have profiled the DNA methylome and transcriptome of matched primary CRC and liver metastasis samples from the same patients. Genome-scale methylation and expression levels were examined using Reduced Representation Bisulfite Sequencing (RRBS) and RNA-Seq, respectively. To investigate DNA methylation and expression patterns, we generated a total of 1.01 × 10 9 RRBS reads and 4.38 × 10 8 RNA-Seq reads from the matched cancer tissues. Here, we describe in detail the sample features, experimental design, methods and bioinformatic pipeline for these epigenetic data. We demonstrate the quality of both the samples and sequence data obtained from the paired samples. The sequencing data obtained from this study will serve as a valuable resource for studying underlying mechanisms of distant metastasis and the utility of epigenetic profiles in cancer metastasis.

Keywords: colorectal cancer; DNA methylation; gene expression; liver metastasis; RRBS; RNA-Seq; matched samples; epigenetics (search for similar items in EconPapers)
JEL-codes: C8 C80 C81 C82 C83 (search for similar items in EconPapers)
Date: 2023
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