The Effects of Plant Growth Regulators on Cell Growth, Protein, Carotenoid, PUFAs and Lipid Production of Chlorella pyrenoidosa ZF Strain

Huanmin Du, Faruq Ahmed, Bin Lin, Zhe Li, Yuhan Huang, Guang Sun, Huan Ding, Chang Wang, Chunxiao Meng and Zhengquan Gao
Additional contact information
Huanmin Du: School of Life Sciences, Shandong University of Technology, Zibo 255049, China
Faruq Ahmed: Centre for Marine Bioproducts Development, Department of Medical Biotechnology, Flinders University, Bedford Park, SA 5042, Australia
Bin Lin: School of Life Sciences, Shandong University of Technology, Zibo 255049, China
Zhe Li: School of Life Sciences, Shandong University of Technology, Zibo 255049, China
Yuhan Huang: School of Life Sciences, Shandong University of Technology, Zibo 255049, China
Guang Sun: School of Life Sciences, Shandong University of Technology, Zibo 255049, China
Huan Ding: School of Life Sciences, Shandong University of Technology, Zibo 255049, China
Chang Wang: School of Life Sciences, Shandong University of Technology, Zibo 255049, China
Chunxiao Meng: School of Life Sciences, Shandong University of Technology, Zibo 255049, China
Zhengquan Gao: School of Life Sciences, Shandong University of Technology, Zibo 255049, China

Energies, 2017, vol. 10, issue 11, 1-23

Abstract: In the present study, eight kinds plant growth regulators—salicylic acid (SA), 1-naphthaleneacetic acid (NAA), gibberellic acid (GA 3 ), 6-benzylaminopurine (6-BA), 2, 4-epi-brassinolide (EBR), abscisic acid (ABA), ethephon (ETH), and spermidine (SPD)—were used to investigate the impact on microalgal biomass, lipid, total soluble protein, carotenoids, and polyunsaturated fatty acids (PUFAS) production of Chlorella pyrenoidosa ZF strain. The results showed the quickest biomass enhancement was induced by 50 mg·L ?1 NAA, with a 6.3-fold increase over the control; the highest protein content was increased by 0.005 mg·L ?1 ETH, which produced 3.5-fold over the control; total carotenoids content was induced most effectively by 1 mg·L ?1 NAA with 3.6-fold higher production than the control; the most efficient elicitor for lipid production was 5 mg·L ?1 GA 3 at 1.9-fold of the control; 0.2 mg·L ?1 ETH induced the abundant production of 1.82 ± 0.23% linoleic acid; 0.65 ± 0.01% linolenic acid was induced by 1 mg·L ?1 NAA; 2.53 ± 0.15% arachidonic acid and 0.44 ± 0.05% docosahexaenoic acid were induced by 5 mg·L ?1 GA 3 . Transcriptional expression levels of seven lipid-related genes, including ACP , BC , FAD , FATA , KAS , MCTK , and SAD , were studied by real-time RT-q-PCR. 5 mg·L ?1 GA 3 was the most effective regulator for transcriptional expressions of these seven genes, producing 23-fold ACP , 31-fold BC , 25-fold FAD , 6-fold KAS , 12-fold MCTK compared with the controls, respectively.

Keywords: Chlorella pyrenoidosa; plant growth regulators; secondary metabolites; gene expression; lipid (search for similar items in EconPapers)
JEL-codes: Q Q0 Q4 Q40 Q41 Q42 Q43 Q47 Q48 Q49 (search for similar items in EconPapers)
Date: 2017
References: View references in EconPapers View complete reference list from CitEc
Citations: View citations in EconPapers (1)

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