Optimization of Reducing Sugar Production from Manihot glaziovii Starch Using Response Surface Methodology

Abdi Hanra Sebayang, Masjuki Haji Hassan, Hwai Chyuan Ong, Surya Dharma, Arridina Susan Silitonga, Fitranto Kusumo, Teuku Meurah Indra Mahlia and Aditiya Harjon Bahar
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Abdi Hanra Sebayang: Department of Mechanical Engineering, Faculty of Engineering, University of Malaya, Kuala Lumpur 50603, Malaysia
Masjuki Haji Hassan: Department of Mechanical Engineering, Faculty of Engineering, University of Malaya, Kuala Lumpur 50603, Malaysia
Hwai Chyuan Ong: Department of Mechanical Engineering, Faculty of Engineering, University of Malaya, Kuala Lumpur 50603, Malaysia
Surya Dharma: Department of Mechanical Engineering, Faculty of Engineering, University of Malaya, Kuala Lumpur 50603, Malaysia
Arridina Susan Silitonga: Department of Mechanical Engineering, Faculty of Engineering, University of Malaya, Kuala Lumpur 50603, Malaysia
Fitranto Kusumo: Department of Mechanical Engineering, Faculty of Engineering, University of Malaya, Kuala Lumpur 50603, Malaysia
Teuku Meurah Indra Mahlia: Department of Mechanical Engineering, Universiti Tenaga Nasional, Kajang 43000, Selangor, Malaysia
Aditiya Harjon Bahar: Department of Mechanical Engineering, Faculty of Engineering, The University of Melbourne, Parkville, VIC 3010, Australia

Energies, 2017, vol. 10, issue 1, 1-13

Abstract: Bioethanol is known as a viable alternative fuel to solve both energy and environmental crises. This study used response surface methodology based on the Box-Behnken experimental design to obtain the optimum conditions for and quality of bioethanol production. Enzymatic hydrolysis optimization was performed with selected hydrolysis parameters, including substrate loading, stroke speed, ?-amylase concentration and amyloglucosidase concentration. From the experiment, the resulting optimum conditions are 23.88% ( w / v ) substrate loading, 109.43 U/g ?-amylase concentration, 65.44 U/mL amyloglucosidase concentration and 74.87 rpm stroke speed, which yielded 196.23 g/L reducing sugar. The fermentation process was also carried out, with a production value of 0.45 g ethanol/g reducing sugar, which is equivalent to 88.61% of ethanol yield after fermentation by using Saccharomyces cerevisiae ( S. cerevisiae ). The physical and chemical properties of the produced ethanol are within the specifications of the ASTM D4806 standard. The good quality of ethanol produced from this study indicates that Manihot glaziovii ( M. glaziovii ) has great potential as bioethanol feedstock.

Keywords: Manihot glaziovii ( M. glaziovii ); optimization; hydrolysis; fermentation; bioethanol; alternative fuel (search for similar items in EconPapers)
JEL-codes: Q Q0 Q4 Q40 Q41 Q42 Q43 Q47 Q48 Q49 (search for similar items in EconPapers)
Date: 2017
References: View references in EconPapers View complete reference list from CitEc
Citations: View citations in EconPapers (8)

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