Methodical Aspects of Biogas Production in Small-Volume Bioreactors in Laboratory Investigations

Agnieszka Kasprzycka and Jan Kuna
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Agnieszka Kasprzycka: Institute of Agrophysics, Polish Academy of Sciences, Doświadczalna 4, 20-290 Lublin, Poland
Jan Kuna: Institute of Agrophysics, Polish Academy of Sciences, Doświadczalna 4, 20-290 Lublin, Poland

Energies, 2018, vol. 11, issue 6, 1-10

Abstract: The aim of this study was to develop a methodology to investigate the biofermentation process in small-volume fermenters. Dark serum bottles with a volume of 100–120 mL, tightly sealed with a rubber septum, were used as bioreactors. The optimum measurement conditions in this type of bioreactor comprise: (i) filling two-thirds of the maximum volume with a suspension; (ii) a 2% bioreactor loading (on a dry basis) and; (iii) the daily equalization of pressure by removing the biogas through the septum pierced with a syringe needle and the intensive mixing of the remaining suspension. The methane yield (quantity and dynamics) obtained in this type of bioreactor is analogous to that of industrial bioreactors or large-scale laboratory bioreactors. The use of small-volume bioreactors that can be incubated will facilitate the preliminary selection of analysed systems and provide an indication of those that should be investigated in large-scale bioreactors.

Keywords: biogas; biofermentation method; energy (search for similar items in EconPapers)
JEL-codes: Q Q0 Q4 Q40 Q41 Q42 Q43 Q47 Q48 Q49 (search for similar items in EconPapers)
Date: 2018
References: View references in EconPapers View complete reference list from CitEc
Citations: View citations in EconPapers (4)

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