Optimization of Batch Dark Fermentation of Chlorella sp. Using Mixed-Cultures for Simultaneous Hydrogen and Butyric Acid Production

Nikannapas Usmanbaha, Rattana Jariyaboon, Alissara Reungsang, Prawit Kongjan and Chen-Yeon Chu
Additional contact information
Nikannapas Usmanbaha: Energy Technology Program, Faculty of Engineering, Prince of Songkla University, Songkhla 90110, Thailand
Rattana Jariyaboon: Bio-Mass Conversion to Energy and Chemicals (Bio-MEC) Research Unit, Faculty of Science and Technology, Prince of Songkla University, Pattani 94000, Thailand
Alissara Reungsang: Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002, Thailand
Prawit Kongjan: Bio-Mass Conversion to Energy and Chemicals (Bio-MEC) Research Unit, Faculty of Science and Technology, Prince of Songkla University, Pattani 94000, Thailand
Chen-Yeon Chu: Master’s Program of Green Energy Science and Technology, Feng Chia University, Taichung 40724, Taiwan

Energies, 2019, vol. 12, issue 13, 1-14

Abstract: This paper reports on the optimum conditions for simultaneous hydrogen and butyric acid production from microalgae ( Chlorella sp.) using enriched anaerobic mixed cultures as inoculum. The fermentation was objectively carried out under acidogenic conditions to achieve butyric acid for further ABE fermentation in solventogenesis stage. The main effects of initial pH (5 and 7), temperature (35 °C and 55 °C), and substrate concentration (40, 60, 80, and 100 g-VS/L) for hydrogen and butyric acid production were evaluated by using batch fermentation experiment. The major effects on hydrogen and butyric acid production are pH and temperature. The highest production of hydrogen and butyric acid was observed at pH 7 and temperature 35 °C. Using initial Chlorella sp. concentration of 80 g-VS/L or 100 g-VS/L at pH 7 and temperature 35 °C could produce hydrogen with an average yield of 22 mL-H 2 /g-VS along with high butyric acid production yield of 0.05 g/g-VS, suggesting that microalgae ( Chlorella sp.) has potential to be converted directly to butyric acid by using acidogenesis under above optimum conditions.

Keywords: microalgae; acidogenesis; hydrogen; butyric acid (search for similar items in EconPapers)
JEL-codes: Q Q0 Q4 Q40 Q41 Q42 Q43 Q47 Q48 Q49 (search for similar items in EconPapers)
Date: 2019
References: View references in EconPapers View complete reference list from CitEc
Citations: View citations in EconPapers (3)

Downloads: (external link)
https://www.mdpi.com/1996-1073/12/13/2529/pdf (application/pdf)
https://www.mdpi.com/1996-1073/12/13/2529/ (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:gam:jeners:v:12:y:2019:i:13:p:2529-:d:244634

Access Statistics for this article

Energies is currently edited by Ms. Agatha Cao

More articles in Energies from MDPI
Bibliographic data for series maintained by MDPI Indexing Manager ().