Assessment of the Effect of Nitrogen Concentration on Fermentation and Selection of a Highly Competitive Saccharomyces cerevisiae Strain for Efficient Ethanol Production

Patricia Portero Barahona, Jesús Martín-Gil, Pablo Martín-Ramos, Ana Briones Pérez and Enrique Javier Carvajal Barriga
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Patricia Portero Barahona: Centro Neotropical para Investigación de la Biomasa (CNIB), Colección de Levaduras Quito Católica (CLQCA), Pontificia Universidad Católica del Ecuador, Avda. 12 de Octubre 1076 y Roca, 17012184, Quito, Ecuador
Jesús Martín-Gil: Agriculture and Forestry Engineering Department, ETSIIAA, Universidad de Valladolid, Avda. de Madrid 44, 34004 Palencia, Spain
Pablo Martín-Ramos: Department of Agricultural and Environmental Sciences, EPS, Instituto de Investigación en Ciencias Ambientales (IUCA), University of Zaragoza, Carretera de Cuarte, s/n, 22071 Huesca, Spain
Ana Briones Pérez: Analytical Chemistry and Food Technology Department, Universidad de Castilla-La Mancha, Avda. Camilo José Cela, 10, 13071 Ciudad Real, Spain
Enrique Javier Carvajal Barriga: Centro Neotropical para Investigación de la Biomasa (CNIB), Colección de Levaduras Quito Católica (CLQCA), Pontificia Universidad Católica del Ecuador, Avda. 12 de Octubre 1076 y Roca, 17012184, Quito, Ecuador

Energies, 2019, vol. 12, issue 13, 1-12

Abstract: The optimum nitrogen concentration for media supplementation and strain dominance are aspects of key importance to the industrial production of ethanol with a view to reducing costs and increasing yields. In this work, these two factors were investigated for four ethanologenic Saccharomyces cerevisiae strains (CLQCA-INT-001, CLQCA-INT-005, CLQCA-10-099, and UCLM 325), selected from the screening of 150 isolates, mostly from Ecuadorian yeast biodiversity. The effect of nitrogen concentration was assessed in terms of cellular growth, glucose consumption and ethanol production, and the yeast strains’ dominance was evaluated in continuous co-fermentation with cellular recycling by mitochondrial DNA analyses. Among the four selected yeast strains under study, CLQCA-INT-005 presented the highest glucose consumption at a nitrogen supplement concentration as low as 0.4 g·L −1 , attaining an ethanol yield of up to 96.72% in 24 h. The same yeast strain was found to be highly competitive, showing a dominance of 80% after four cycles of fermentation in co-culture. Thus, CLQCA-INT-005 may be deemed as a very promising candidate to be used both at pilot-plant scale and at industrial scale cellulosic ethanol production.

Keywords: ethanol; fermentation; nitrogen; Saccharomyces cerevisiae (search for similar items in EconPapers)
JEL-codes: Q Q0 Q4 Q40 Q41 Q42 Q43 Q47 Q48 Q49 (search for similar items in EconPapers)
Date: 2019
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