Improvement of Bioethanol Production from Sweet Sorghum Juice under Very High Gravity Fermentation: Effect of Nitrogen, Osmoprotectant, and Aeration

Niphaphat Phukoetphim, Pachaya Chan-u-tit, Pattana Laopaiboon and Lakkana Laopaiboon
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Niphaphat Phukoetphim: Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002, Thailand
Pachaya Chan-u-tit: Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002, Thailand
Pattana Laopaiboon: Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002, Thailand
Lakkana Laopaiboon: Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002, Thailand

Energies, 2019, vol. 12, issue 19, 1-13

Abstract: To improve ethanol production fermentation efficiency from sweet sorghum juice under a very high gravity (VHG, 280 g/L of total sugar) condition by Saccharomyces cerevisiae NP01, dried spent yeast (DSY), yeast extract, and glycine concentrations were optimized using an L 9 (3 4 ) orthogonal array design. The results showed that the order of influence on the ethanol concentration ( P E ) was yeast extract > glycine > DSY. The optimal nutrient concentrations for ethanol production were determined as follows: yeast extract, 3; DSY, 4; and glycine, 5 g/L. When a verification experiment under the projected optimal conditions was done, the P , ethanol yield ( Y p/s ), and ethanol productivity ( Q p ) values were 120.1 g/L, 0.47, and 2.50 g/L·h, respectively. These values were similar to those of the positive control experiment with yeast extract supplementation at 9 g/L. The yeast viability under the optimal condition was higher than that of the control experiment. To improve sugar utilization and ethanol production, aeration at 2.5 vvm for 4 h was applied under the optimal nutrient supplementation. The P , Y p/s , and Q p values were significantly increased to 134.3 g/L, 0.50, and 2.80 g/L·h, respectively.

Keywords: very high gravity (VHG); nitrogen source; osmoprotectant; aeration; sweet sorghum; Saccharomyces cerevisiae (search for similar items in EconPapers)
JEL-codes: Q Q0 Q4 Q40 Q41 Q42 Q43 Q47 Q48 Q49 (search for similar items in EconPapers)
Date: 2019
References: View references in EconPapers View complete reference list from CitEc
Citations: View citations in EconPapers (1)

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