Rhizomucor miehei Lipase Supported on Inorganic Solids, as Biocatalyst for the Synthesis of Biofuels: Improving the Experimental Conditions by Response Surface Methodology

Juan Calero, Diego Luna, Carlos Luna, Felipa M. Bautista, Beatriz Hurtado, Antonio A. Romero, Alejandro Posadillo and Rafael Estevez
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Juan Calero: Departamento de Química Orgánica/Universidad de Córdoba, Campus de Rabanales, Ed. Marie Curie, 14014 Córdoba, Spain
Diego Luna: Departamento de Química Orgánica/Universidad de Córdoba, Campus de Rabanales, Ed. Marie Curie, 14014 Córdoba, Spain
Carlos Luna: Departamento de Química Orgánica/Universidad de Córdoba, Campus de Rabanales, Ed. Marie Curie, 14014 Córdoba, Spain
Felipa M. Bautista: Departamento de Química Orgánica/Universidad de Córdoba, Campus de Rabanales, Ed. Marie Curie, 14014 Córdoba, Spain
Beatriz Hurtado: Departamento de Química Orgánica/Universidad de Córdoba, Campus de Rabanales, Ed. Marie Curie, 14014 Córdoba, Spain
Antonio A. Romero: Departamento de Química Orgánica/Universidad de Córdoba, Campus de Rabanales, Ed. Marie Curie, 14014 Córdoba, Spain
Alejandro Posadillo: Seneca Green Catalyst S.L., Campus de Rabanales, 14014 Córdoba, Spain
Rafael Estevez: Departamento de Química Orgánica/Universidad de Córdoba, Campus de Rabanales, Ed. Marie Curie, 14014 Córdoba, Spain

Energies, 2019, vol. 12, issue 5, 1-15

Abstract: Two inorganic solids have been evaluated as supports of Lipozyme RM IM, a Rhizomucor miehei lipase immobilized on a macroporous anion exchange resin, in order to improve its application as a biocatalyst in the synthesis of biofuels. The experimental conditions have been optimized to get the selective transesterification of sunflower oil, by using a multi-factorial design based on the response surface methodology (RSM). In this way, the effects of several reaction parameters on the selective ethanolysis of triglycerides to produce Ecodiesel, a biodiesel-like biofuel constitute by one mole of monoglyceride (MG) and two moles of fatty acid ethyl ester (FAEE), have been evaluated. Thus, it was obtained that a 6:1 oil/ethanol molar ratio, 0.215 g of biocatalyst supported in silica-gel (0.015 g Lipase/0.2 g silica-gel), 50 µL of 10 N NaOH, together with previous optimized reaction parameters, 35 °C reaction temperature and 120 min of reaction time, gave the best results (conversions around 70%; selectivity around 65%; kinematic viscosities about 9.3 mm 2 /s) in the reaction studied. Besides, Lipozyme RM IM, supported on silica-gel, biocatalyst exhibited a very good stability, remaining its activity even after 15 cycles.

Keywords: biodiesel; Ecodiesel; selective ethanolysis; sunflower oil; Lipozyme RM IM; Rhizomucor miehei; ANOVA method; response surface methodology (search for similar items in EconPapers)
JEL-codes: Q Q0 Q4 Q40 Q41 Q42 Q43 Q47 Q48 Q49 (search for similar items in EconPapers)
Date: 2019
References: View references in EconPapers View complete reference list from CitEc
Citations: View citations in EconPapers (2)

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