Enhancing Hydrogen Production from Chlorella sp. Biomass by Pre-Hydrolysis with Simultaneous Saccharification and Fermentation (PSSF)

Tran Thi Giang, Siriporn Lunprom, Qiang Liao, Alissara Reungsang and Apilak Salakkam
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Tran Thi Giang: Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002, Thailand
Siriporn Lunprom: Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002, Thailand
Qiang Liao: Key Laboratory of Low-grade Energy Utilization Technologies and Systems, Chongqing University, Ministry of Education, Chongqing 400044, China
Alissara Reungsang: Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002, Thailand
Apilak Salakkam: Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002, Thailand

Energies, 2019, vol. 12, issue 5, 1-14

Abstract: Simultaneous saccharification and fermentation (SSF) and pre-hydrolysis with SSF (PSSF) were used to produce hydrogen from the biomass of Chlorella sp. SSF was conducted using an enzyme mixture consisting of 80 filter paper unit (FPU) g-biomass −1 of cellulase, 92 U g-biomass −1 of amylase, and 120 U g-biomass −1 of glucoamylase at 35 °C for 108 h. This yielded 170 mL-H 2 g-volatile-solids −1 (VS), with a productivity of 1.6 mL-H 2 g-VS −1 h −1 . Pre-hydrolyzing the biomass at 50 °C for 12 h resulted in the production of 1.8 g/L of reducing sugars, leading to a hydrogen yield (HY) of 172 mL-H 2 g-VS −1 . Using PSSF, the fermentation time was shortened by 36 h in which a productivity of 2.4 mL-H 2 g-VS −1 h −1 was attained. To the best of our knowledge, the present study is the first report on the use of SSF and PSSF for hydrogen production from microalgal biomass, and the HY obtained in the study is by far the highest yield reported. Our results indicate that PSSF is a promising process for hydrogen production from microalgal biomass.

Keywords: renewable energy; microalgal biomass; pretreatment; enzymatic hydrolysis; dark fermentation (search for similar items in EconPapers)
JEL-codes: Q Q0 Q4 Q40 Q41 Q42 Q43 Q47 Q48 Q49 (search for similar items in EconPapers)
Date: 2019
References: View references in EconPapers View complete reference list from CitEc
Citations: View citations in EconPapers (5)

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