Electro-Fenton Based Technique to Enhance Cell Harvest and Lipid Extraction from Microalgae

Shuai Zhang, Yuyong Hou, Zhiyong Liu, Xiang Ji, Di Wu, Weijie Wang, Dongyuan Zhang, Wenya Wang, Shulin Chen and Fangjian Chen
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Shuai Zhang: Tianjin Key Laboratory of Industrial Biosystems and Bioprocess Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China
Yuyong Hou: Tianjin Key Laboratory of Industrial Biosystems and Bioprocess Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China
Zhiyong Liu: Tianjin Key Laboratory of Industrial Biosystems and Bioprocess Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China
Xiang Ji: School of Life Science and Technology, Inner Mongolia University of Science and Technology, Baotou 014010, China
Di Wu: School of Life Science and Technology, Inner Mongolia University of Science and Technology, Baotou 014010, China
Weijie Wang: College of Life Science, North China University of Science and Technology, Tangshan 063210, China
Dongyuan Zhang: Tianjin Key Laboratory of Industrial Biosystems and Bioprocess Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China
Wenya Wang: College of Life Science and Techenology, Beijing University of Chemical Technology, Beijing 100029, China
Shulin Chen: Department of Biological Systems Engineering, Washington State University, Pullman, WA 99164-6120, USA
Fangjian Chen: Tianjin Key Laboratory of Industrial Biosystems and Bioprocess Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China

Energies, 2020, vol. 13, issue 15, 1-14

Abstract: Currently, lipid extraction remains a major bottleneck in microalgae technology for biofuel production. In this study, an effective and easily controlled cell wall disruption method based on electro-Fenton reaction was used to enhance lipid extraction from the wet biomass of Nannochloropsis oceanica IMET1. The results showed that 1.27 mM of hydroxide radical (HO•) was generated under the optimal conditions with 9.1 mM FeSO 4 in a 16.4 mA·cm −2 current density for 37.0 min. After the electro-Fenton treatment, the neutral lipid extraction yield of microalgae (~155 mg) increased from 40% to 87.5%, equal to from 12.2% to 26.7% dry cell weight (DCW). In particular, the fatty acid composition remained stable. The cell wall disruption and lipid extraction processes were displayed by the transmission electron microscope (TEM) and fluorescence microscopy (FM) observations, respectively. Meanwhile, the removal efficiency of algal cells reached 85.2% within 2 h after the reaction was terminated. Furthermore, the biomass of the microalgae cultured in the electrolysis wastewater treated with fresh nutrients reached 3 g/L, which is 12-fold higher than that of the initial after 24 days. These finds provided an economic and efficient method for lipid extraction from wet microalgae, which could be easily controlled by current magnitude regulation.

Keywords: lipid extraction; electro-Fenton reaction; cell wall disruption; microalgae (search for similar items in EconPapers)
JEL-codes: Q Q0 Q4 Q40 Q41 Q42 Q43 Q47 Q48 Q49 (search for similar items in EconPapers)
Date: 2020
References: View references in EconPapers View complete reference list from CitEc
Citations: View citations in EconPapers (3)

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