Saccharification Yield through Enzymatic Hydrolysis of the Steam-Exploded Pinewood

Merve Nazli Borand, Asli Isler Kaya and Filiz Karaosmanoglu
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Merve Nazli Borand: Energy Systems Engineering Department, Faculty of Engineering, Merkez Campus, Yalova University, 77100 Yalova, Turkey
Asli Isler Kaya: Chemical Engineering Department, Faculty of Chemical and Metallurgical Engineering, Ayazaga Campus Istanbul Technical University, 34467 Istanbul, Turkey
Filiz Karaosmanoglu: Chemical Engineering Department, Faculty of Chemical and Metallurgical Engineering, Ayazaga Campus Istanbul Technical University, 34467 Istanbul, Turkey

Energies, 2020, vol. 13, issue 17, 1-12

Abstract: Pressure, temperature, and retention time are the most studied parameters in steam explosion pretreatment. However, this work aimed to fix these parameters and to evaluate the influences of several less investigated steam explosion parameters on the saccharification yield in hydrolysis. In this study, firstly, pinewood samples smaller than 200 µm were treated with steam explosion at 190 °C for 10 min. The variable parameters were biomass loading, N 2 pressure, and release time. Steam-exploded samples were hydrolyzed with the Trichoderma reesei enzyme for saccharification for 72 h. The sugar content of the resultant products was analyzed to estimate the yield of sugars (such as glucose, xylose, galactose, mannose, and arabinose). The best glucose yield in the pulp was achieved with 4 g of sample, N 2 pressure of 0.44 MPa, and short release time (22 s). These conditions gave a glucose yield of 97.72% in the pulp, and the xylose, mannose, galactose, and arabinose yields in the liquid fraction were found to be 85.59%, 87.76%, 86.43%, and 90.3%, respectively.

Keywords: lignocellulosic biomass; hydrothermal pretreatment; enzymatic hydrolysis; sugar yield; high-performance liquid chromatography (HPLC) analysis (search for similar items in EconPapers)
JEL-codes: Q Q0 Q4 Q40 Q41 Q42 Q43 Q47 Q48 Q49 (search for similar items in EconPapers)
Date: 2020
References: View references in EconPapers View complete reference list from CitEc
Citations: View citations in EconPapers (1)

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