Impacts of Initial Sugar, Nitrogen and Calcium Carbonate on Butanol Fermentation from Sugarcane Molasses by Clostridium beijerinckii

Patthranit Narueworanon, Lakkana Laopaiboon, Niphaphat Phukoetphim and Pattana Laopaiboon
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Patthranit Narueworanon: Graduate School, Khon Kaen University, Khon Kaen 40002, Thailand
Lakkana Laopaiboon: Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002, Thailand
Niphaphat Phukoetphim: Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002, Thailand
Pattana Laopaiboon: Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002, Thailand

Energies, 2020, vol. 13, issue 3, 1-19

Abstract: Low-cost nitrogen sources, i.e., dried spent yeast (DSY), rice bran (RB), soybean meal (SM), urea and ammonium sulfate were used for batch butanol fermentation from sugarcane molasses by Clostridium beijerinckii TISTR 1461 under anaerobic conditions. Among these five low-cost nitrogen sources, DSY at 1.53 g/L (nitrogen content equal to that of 1 g/L of yeast extract) was found to be the most suitable. At an initial sugar level of 60 g/L, the maximum butanol concentration ( P B ), productivity ( Q B ) and yield ( Y B / S ) were 11.19 g/L, 0.23 g/L·h and 0.31 g/g, respectively. To improve the butanol production, the concentrations of initial sugar, DSY and calcium carbonate were varied using response surface methodology (RSM) based on Box–Behnken design. It was found that the optimal conditions for high butanol production were initial sugar, 50 g/L; DSY, 6 g/L and calcium carbonate, 6.6 g/L. Under these conditions, the highest experimental P B , Q B and Y B / S values were 11.38 g/L, 0.32 g/L·h and 0.40 g/g, respectively with 50% sugar consumption ( SC ). The P B with neither DSY nor CaCO 3 was only 8.53 g/L. When an in situ gas stripping system was connected to the fermenter to remove butanol produced during the fermentation, the P B was increased to 15.33 g/L, whereas the Y B / S (0.39 g/g) was not changed. However, the Q B was decreased to 0.21 g/L·h with 75% SC .

Keywords: butanol; molasses; Clostridium beijerinckii; low-cost nitrogen sources; response surface methodology (RSM); gas stripping (search for similar items in EconPapers)
JEL-codes: Q Q0 Q4 Q40 Q41 Q42 Q43 Q47 Q48 Q49 (search for similar items in EconPapers)
Date: 2020
References: View complete reference list from CitEc
Citations: View citations in EconPapers (2)

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