Enzymatic Saccharification of Laminaria japonica by Cellulase for the Production of Reducing Sugars

Eun Young Park and Jung Kyu Park
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Eun Young Park: Department of Biomedical Laboratory Science, Shinhan University, Uijeongbu-si 11644, Korea
Jung Kyu Park: Department of Computer Software Engineering, Changshin University, Changwon-si 51352, Korea

Energies, 2020, vol. 13, issue 3, 1-9

Abstract: Enzymatic saccharification of Laminaria japonica seaweed biomass was optimized by four independent factors (enzyme dose, hydrolysis time, pH, and temperature) using response surface methodology (RSM). To confirm the significance of the quadratic model, an analysis of variance (ANOVA) was performed, and the F -value of 8.76 showed that the regression model was highly significant (≤0.1%). In the accuracy study, average recoveries were in the range of 97.00% to 98.32%. The optimum experimental conditions were an enzyme dose of 8.2%, a hydrolysis time of 26 h, a pH of 4.1, and a temperature of 43 °C. Temperature was the most important factor in the enzymatic saccharification. A relatively low temperature and short hydrolysis time were shown to improve the yield of reducing sugars.

Keywords: enzymatic hydrolysis; enzymatic saccharification; reducing-sugars yield; response surface methodology (search for similar items in EconPapers)
JEL-codes: Q Q0 Q4 Q40 Q41 Q42 Q43 Q47 Q48 Q49 (search for similar items in EconPapers)
Date: 2020
References: View references in EconPapers View complete reference list from CitEc
Citations: View citations in EconPapers (1)

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