Rhodotorula toruloides Single Cell Oil Production Using Eucalyptus urograndis Hemicellulose Hydrolysate as a Carbon Source

Helberth Júnnior Santos Lopes, Nemailla Bonturi and Everson Alves Miranda
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Helberth Júnnior Santos Lopes: Department of Materials and Bioprocess Engineering, School of Chemical Engineering, State University of Campinas, Campinas SP 13083-852, Brazil
Nemailla Bonturi: Institute of Technology, University of Tartu, 50411 Tartu, Estonia
Everson Alves Miranda: Department of Materials and Bioprocess Engineering, School of Chemical Engineering, State University of Campinas, Campinas SP 13083-852, Brazil

Energies, 2020, vol. 13, issue 4, 1-11

Abstract: Microbial oil is a potential substitute for vegetable oils in the biodiesel industry. Efforts to obtain cheap carbon sources for the cultivation of lipid-producing microorganisms comprise an active research area. This work aimed to extract the hemicellulose fraction from Eucalyptus uograndis and to use its hydrolysate as a carbon source for Rhodotorula toruloides (an oleaginous yeast) cultivation for microbial oil production. Hemicellulose hydrothermal extractions were performed at different temperatures, times, and ratios of solid to liquid (S/L). Temperature and time showed a stronger effect on the solubilization of hemicellulose. Hemicellulose extraction at 155 °C, 195 min, and an S/L ratio of 1/2 resulted in a hydrolysate with a xylose content of 37.0 g/l. R. toruloides cultivation in this hydrolysate showed that initial pH had a strong influence on cell growth. At an initial pH of 6.2, cells grew to 6.0 g/l of biomass with a lipid content of 50%. Therefore, we believe that E. urograndis hemicellulose hydrolysate could be a potential substrate for R. toruloides for lipid production based on the biorefinery concept.

Keywords: Eucalyptus urograndis; Rhodotorula toruloides; hemicellulose; hydrolysate; acetic acid; single cell oil (search for similar items in EconPapers)
JEL-codes: Q Q0 Q4 Q40 Q41 Q42 Q43 Q47 Q48 Q49 (search for similar items in EconPapers)
Date: 2020
References: View references in EconPapers View complete reference list from CitEc
Citations: View citations in EconPapers (3)

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