Ethanol Production from High Solid Loading of Rice Straw by Simultaneous Saccharification and Fermentation in a Non-Conventional Reactor

Inês C. Roberto, Rafael C. A. Castro, João Paulo A. Silva and Solange I. Mussatto
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Inês C. Roberto: Departamento de Biotecnologia, Escola de Engenharia de Lorena, Universidade de São Paulo, Lorena/SP 12602-810, Brazil
Rafael C. A. Castro: Departamento de Biotecnologia, Escola de Engenharia de Lorena, Universidade de São Paulo, Lorena/SP 12602-810, Brazil
João Paulo A. Silva: Departamento de Engenharia Química, Escola de Engenharia de Lorena, Universidade de São Paulo, Lorena/SP 12602-810, Brazil
Solange I. Mussatto: Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kemitorvet, Building 220, 2800 Kongens Lyngby, Denmark

Energies, 2020, vol. 13, issue 8, 1-17

Abstract: Simultaneous saccharification and fermentation (SSF) at high solid loading is a potential approach to improve the economic feasibility of cellulosic ethanol. In this study, SSF using high loading of rice straw was assessed using a vertical ball mill reactor. First, the conditions of temperature and number of glass spheres were optimized at 8% ( w / v ) initial solids (41.5 °C, 18 spheres). Then, assays were carried out at higher solid loadings (16% and 24% w / v ). At 8% or 16% solids, the fermentation efficiency was similar (η F ~75%), but the ethanol volumetric productivity (Q P ) reduced from 1.50 to 1.14 g/L.h. By increasing the solids to 24%, the process was strongly affected (η F = 40% and Q P = 0.7 g/L.h). To overcome this drawback, three different feeding profiles of 24% pre-treated rice straw were investigated. Gradual feeding of the substrate (initial load of 16% with additions of 4% at 10 and 24 h) and an inoculum level of 3 g/L resulted in a high ethanol titer (52.3 g/L) with Q P of 1.1 g/L.h and η F of 67%. These findings demonstrated that using a suitable fed-batch feeding strategy helps to overcome the limitations of SSF in batch mode caused by the use of high solid content.

Keywords: ethanol; simultaneous saccharification and fermentation; rice straw; vertical ball mill reactor; Kluyveromyces marxianus (search for similar items in EconPapers)
JEL-codes: Q Q0 Q4 Q40 Q41 Q42 Q43 Q47 Q48 Q49 (search for similar items in EconPapers)
Date: 2020
References: View references in EconPapers View complete reference list from CitEc
Citations: View citations in EconPapers (4)

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