Sequential Hydrothermal HCl Pretreatment and Enzymatic Hydrolysis of Saccharina japonica Biomass

Eun-Young Park and Jung-Kyu Park
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Eun-Young Park: Rinascita College of Liberal Arts and Sciences, Shinhan University, Uijeongbu-si 11644, Korea
Jung-Kyu Park: Department of Computer Software Engineering, Changshin University, Changwon-si 51352, Korea

Energies, 2021, vol. 14, issue 23, 1-9

Abstract: This study investigated the production of fermentable sugars from carbohydrate-rich macroalgae Saccharina japonica using sequential hydrolysis (hydrothermal acid pretreatment and enzymatic hydrolysis) to determine the maximum reducing sugar yield (RS y ). The sequential hydrolysis was predicted by three independent variables (temperature, time, and HCl concentration) using response surface methodology (RSM). Enzymatic hydrolysis (8.17% v/w b i o m a s s Celluclast ® 1.5 L, 26.4 h, 42.6 °C) was performed after hydrothermal acid pretreatment under predicted conditions (143.6 °C, 22 min, and 0.108 N HCl concentration). Using this experimental procedure, the yields of hydrothermal acid pretreatment, enzymatic hydrolysis, and sequential hydrolysis were 115.6 ± 0.4 mg/g, 117.7 ± 0.3 mg/g, and 183.5 ± 0.6 mg/g, respectively. Our results suggested that sequential hydrolysis of hydrothermal acid pretreatment and enzymatic hydrolysis was more efficient than their single treatment.

Keywords: sequential hydrolysis; hydrothermal acid pretreatment; enzymatic hydrolysis; reducing sugar yield; biomass (search for similar items in EconPapers)
JEL-codes: Q Q0 Q4 Q40 Q41 Q42 Q43 Q47 Q48 Q49 (search for similar items in EconPapers)
Date: 2021
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