Optical Evaluation of Effects of Energy Substrates on PHB Accumulation for Bioplastic Production

Alicja Staśczak, Hanna Langer-Macioł, Karolina Widzisz, Wiktoria Śliwińska, Kinga Lucińska, Przemysław Wencel, Barbara Strózik, Mariusz Frąckiewicz, Piotr Skupin (), Dariusz Choiński and Sebastian Student
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Alicja Staśczak: Department of Automatic Control and Robotics, Silesian University of Technology, Akademicka 16, 44-100 Gliwice, Poland
Hanna Langer-Macioł: Department of Automatic Control and Robotics, Silesian University of Technology, Akademicka 16, 44-100 Gliwice, Poland
Karolina Widzisz: Department of Automatic Control and Robotics, Silesian University of Technology, Akademicka 16, 44-100 Gliwice, Poland
Wiktoria Śliwińska: Department of Automatic Control and Robotics, Silesian University of Technology, Akademicka 16, 44-100 Gliwice, Poland
Kinga Lucińska: Department of Automatic Control and Robotics, Silesian University of Technology, Akademicka 16, 44-100 Gliwice, Poland
Przemysław Wencel: Department of Automatic Control and Robotics, Silesian University of Technology, Akademicka 16, 44-100 Gliwice, Poland
Barbara Strózik: Department of Automatic Control and Robotics, Silesian University of Technology, Akademicka 16, 44-100 Gliwice, Poland
Mariusz Frąckiewicz: Department of Data Science and Engineering, Silesian University of Technology, Akademicka 16, 44-100 Gliwice, Poland
Piotr Skupin: Department of Automatic Control and Robotics, Silesian University of Technology, Akademicka 16, 44-100 Gliwice, Poland
Dariusz Choiński: Department of Automatic Control and Robotics, Silesian University of Technology, Akademicka 16, 44-100 Gliwice, Poland
Sebastian Student: Biotechnology Center, Silesian University of Technology, Krzywoustego 8, 44-100 Gliwice, Poland

Energies, 2022, vol. 15, issue 22, 1-16

Abstract: To date, hundreds of millions tons of plastics has been produced worldwide. Their production and disposal are associated with high pollution and carbon release into the atmosphere. A more environmentally friendly alternative is bioplastics, and the most popular is polyhydroxybutyrate (PHB) polymer. Large amounts of PHB can be obtained from activated sludge where used cooking oil or other industrial waste can be used as potential substrates. In this work, efficient bioplastic production strategies are studied, and the considered substrate is a mixture of oil and peptone. Pseudomonas fluorescens bacteria are used to accumulate PHB, and the cultivation of microorganisms is carried out in batch and continuous-flow bioreactors. Microscopic observations and laboratory essays are performed to confirm presence of PHB and other key parameters. The obtained results allow us to determine the optimal feeding strategy.

Keywords: polyhydroxybutyrate (PHB); polyhydroxyalkanoates (PHA); bioplastic; Pseudomonas fluorescens (search for similar items in EconPapers)
JEL-codes: Q Q0 Q4 Q40 Q41 Q42 Q43 Q47 Q48 Q49 (search for similar items in EconPapers)
Date: 2022
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