Microencapsulation of Lipases Produced by Dripping and Jet Break-Up for Biodiesel Production

Boris Guzmán-Martínez, Roberto Limas-Ballesteros (), Jin An Wang, Liliana Alamilla-Beltrán, Lifang Chen () and Luis Enrique Noreña
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Boris Guzmán-Martínez: Laboratorio de Investigación en Ingeniería Química Ambiental, ESIQIE, Instituto Politécnico Nacional, Col. Zacatenco, Mexico City C.P. 07738, Mexico
Roberto Limas-Ballesteros: Laboratorio de Investigación en Ingeniería Química Ambiental, ESIQIE, Instituto Politécnico Nacional, Col. Zacatenco, Mexico City C.P. 07738, Mexico
Jin An Wang: Laboratorio de Catálisis y Materiales, ESIQIE, Instituto Politécnico Nacional, Col. Zacatenco, Mexico City C.P. 07738, Mexico
Liliana Alamilla-Beltrán: Laboratorio de Microencapsulación: Interacciones, Estructura y Función, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Av. Wilfrido Massieu S/N, Mexico City C.P. 07739, Mexico
Lifang Chen: Departamento de Ingeniería Química Petrolera, ESIQIE, Instituto Politécnico Nacional, Col. Zacatenco, Mexico City C.P. 07738, Mexico
Luis Enrique Noreña: Departamento de Ciencias Básicas, Universidad Autónoma Metropolitana-Azcapotzalco, Av. San Pablo 180, Col. Reynosa Tamaulipas, Mexico City C.P. 02200, Mexico

Energies, 2022, vol. 15, issue 24, 1-18

Abstract: A high-performance and scalable lipase immobilization method using a dipping and jet break-up technique was reported for the production of microcapsule biocatalysts with an entrapped cascade of lipase enzyme. The lipase from Candida antarctica (CALb) recombinant Aspergillus oryzae and from the vegetal of Jatropha curcas L. (var. Sevangel) in Morelos State of Mexico were entrapped by mixing with a sodium alginate biopolymer at different concentrations. The obtained microcapsules were hardened in a CaCl 2 solution, aiming at developing Ca 2+ alginate microbeads with sizes mostly from 220 to 300 μm. The relationship between the process variables with the shape and size of the alginate drops before and after the gelation was established with aid of optical image analysis. The results showed that a critical Ohnesorge number (Oh) > 0.24 was required to form spherical microencapsulated beads. The biodiesel production via esterification/transesterification reaction was performed using the crude Jatropha curcas L. oil as feedstock in a batch reactor using lipase microcapsules as biocatalysts. Under the optimal reaction condition (ethanol-to-oil mass ratio: 10; water content 9.1 wt%, microencapsulated biocatalyst mass: 5.25 g, reaction temperature: 35 °C, pH of reaction mixture 7.5, stirring force 6 g), an approximately 95% fatty acid ethyl esters (FAEE) yield could be obtained. The biodiesel obtained from this work completely satisfied with the related ASTM D6751 and EN14214 standards. The microencapsulation technique reported herein allows the production of lipase microcapsules on a continuous large scale with the characteristics required for sustainable biofuel production and it can be also applied in other fields such as food processing and the pharmaceutical industry.

Keywords: biodiesel; transesterification; microencapsulated lipase; Jatropha curcas L. oil; biocatalysts (search for similar items in EconPapers)
JEL-codes: Q Q0 Q4 Q40 Q41 Q42 Q43 Q47 Q48 Q49 (search for similar items in EconPapers)
Date: 2022
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