Immobilizing Enzymes on a Commercial Polymer: Performance Analysis of a GOx-Laccase Based Enzymatic Biofuel Cell Assembly

Dario Pelosi, Linda Barelli, Nicolò Montegiove, Eleonora Calzoni, Alessio Cesaretti, Alessandro Di Michele, Carla Emiliani and Luca Gammaitoni
Additional contact information
Dario Pelosi: Department of Engineering, University of Perugia, Via G. Duranti 93, 06125 Perugia, Italy
Linda Barelli: Department of Engineering, University of Perugia, Via G. Duranti 93, 06125 Perugia, Italy
Nicolò Montegiove: Department of Chemistry, Biology and Biotechnology, University of Perugia, Via del Giochetto, 06123 Perugia, Italy
Eleonora Calzoni: Department of Chemistry, Biology and Biotechnology, University of Perugia, Via del Giochetto, 06123 Perugia, Italy
Alessio Cesaretti: Department of Chemistry, Biology and Biotechnology, University of Perugia, Via del Giochetto, 06123 Perugia, Italy
Alessandro Di Michele: Department of Physics and Geology, University of Perugia, Via Pascoli, 06123 Perugia, Italy
Carla Emiliani: Department of Chemistry, Biology and Biotechnology, University of Perugia, Via del Giochetto, 06123 Perugia, Italy
Luca Gammaitoni: Department of Physics and Geology, University of Perugia, Via Pascoli, 06123 Perugia, Italy

Energies, 2022, vol. 15, issue 6, 1-12

Abstract: Enzymatic Biofuel Cell (EBC) represents a promising green source since it is capable of harvesting electricity from renewable and abundantly available biofuels using enzymes as catalysts. Nevertheless, nowadays long-term stability and low power output are currently the main concerns. To this end, several research studies focus on using complex tridimensional and highly expensive nanostructures as electrode support for enzymes. This increases cell performance whilst drastically reducing the economic feasibility needed for industrial viability. Thus, this paper analyzes a novel flow-based EBC consisting of covalent immobilized GOx (bioanode) and Laccase (biocathode) on a commercial flat conductive polymer. A suitable immobilization technique based on covalent ligands is carried out to enhance EBC durability. The experimental characterization demonstrates that the cell generates power over three weeks, reaching 590 mV and 2.41 µW cm −2 as maximum open circuit voltage and power density, respectively. The most significant contributions of this configuration are definitely ease of implementation, low cost, high scalability, and reproducibility. Therefore, such a design can be considered a step forward in the viable EBC industrialization process for a wide range of applications.

Keywords: covalent immobilization; direct electron transfer; enzymatic biofuel cell; enzymes; laccase; glucose oxidase; electrochemical stability; green power; low-cost polymer; power generation (search for similar items in EconPapers)
JEL-codes: Q Q0 Q4 Q40 Q41 Q42 Q43 Q47 Q48 Q49 (search for similar items in EconPapers)
Date: 2022
References: View references in EconPapers View complete reference list from CitEc
Citations:

Downloads: (external link)
https://www.mdpi.com/1996-1073/15/6/2182/pdf (application/pdf)
https://www.mdpi.com/1996-1073/15/6/2182/ (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:gam:jeners:v:15:y:2022:i:6:p:2182-:d:772887

Access Statistics for this article

Energies is currently edited by Ms. Agatha Cao

More articles in Energies from MDPI
Bibliographic data for series maintained by MDPI Indexing Manager ().