Characterization of the Ensemble of Lignin-Remodeling DyP-Type Peroxidases from Streptomyces coelicolor A3(2)

Hegne Pupart, Piia Jõul, Melissa Ingela Bramanis and Tiit Lukk ()
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Hegne Pupart: Department of Chemistry and Biotechnology, Tallinn University of Technology, Akadeemia tee 15, 12618 Tallinn, Estonia
Piia Jõul: Department of Chemistry and Biotechnology, Tallinn University of Technology, Akadeemia tee 15, 12618 Tallinn, Estonia
Melissa Ingela Bramanis: Department of Chemistry and Biotechnology, Tallinn University of Technology, Akadeemia tee 15, 12618 Tallinn, Estonia
Tiit Lukk: Department of Chemistry and Biotechnology, Tallinn University of Technology, Akadeemia tee 15, 12618 Tallinn, Estonia

Energies, 2023, vol. 16, issue 3, 1-15

Abstract: Lignin is Nature’s major source of aromatic chemistry and is by many seen as the green entry-point alternative to the fossil-based chemical industry. Due to its chemically recalcitrant structure, the utilization of lignin is challenging, wherein enzymes might be the key to overcome this challenge. Here, we focus on the characterization of dye-decolorizing peroxidases from Streptomyces coelicolor A3(2) ( Sc DyPs) in the context of enzymatic modification of organosolv lignins from aspen and Miscanthus × giganteus . In this study, we show that the Sc DyPB can remodel organosolv lignins from grassy biomass, leading to higher molecular weight species, while Sc DyPAs can deconstruct hardwood lignin, leading to an overall reduction in its molecular weight. Additionally, we show that Sc DyPB is effective in polymerizing low-molecular-weight phenolics, leading to their removal from the solution.

Keywords: lignin remodeling; DyP-type peroxidases; Actinobacteria (search for similar items in EconPapers)
JEL-codes: Q Q0 Q4 Q40 Q41 Q42 Q43 Q47 Q48 Q49 (search for similar items in EconPapers)
Date: 2023
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