Experimental Study of Bio-Hydrogen Production by Clostridium beijerinckii from Different Substrates

Venko Beschkov (), Tsvetomila Parvanova-Mancheva and Evgenia Vasileva
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Venko Beschkov: Laboratory of Bioengineering, Institute of Chemical Engineering, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria
Tsvetomila Parvanova-Mancheva: Laboratory of Bioengineering, Institute of Chemical Engineering, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria
Evgenia Vasileva: Laboratory of Bioengineering, Institute of Chemical Engineering, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria

Energies, 2023, vol. 16, issue 6, 1-13

Abstract: Glucose, alcohol stillage and glycerol were used as substrates for bio-hydrogen production by the newly isolated strain Clostridium beijerinckii 6A1 under batch conditions. High molar yields of hydrogen from the studied organic substrates were observed. There was a neat difference in the metabolic pathways of substrate digestion when hexose-based substrate or glycerol were used. The products of glycerol digestion showed that a pathway with no formic acid formation as intermediate was probable. In this case, considerable concentrations of acetic and propionic acid (up to 6 g dm ?3 ) and small amounts of butanol were observed after 48 h. When glucose or hexose-based substrates were used, considerable amounts of formic acid (up to 6 g dm ?3 ), i.e., the pathway proposed for Clostridia mixed cultures, were appropriate for the observed process of hydrogen release. For these substrates, considerable amounts of propionic acid in concentrations up to 1 g dm ?3 were observed. That is why the pathway proposed for mixed cultures seemed more appropriate for our experiments carried out with hexose-based substrates. When hexoses were used, substrate digestion stopped the formation of acetic acid, propionic acid and ethanol. Probably, these intermediates are inhibitors to the further digestion to other products.

Keywords: bio-hydrogen; dark fermentation; Clostridium beijerinckii; hexoses; glycerol (search for similar items in EconPapers)
JEL-codes: Q Q0 Q4 Q40 Q41 Q42 Q43 Q47 Q48 Q49 (search for similar items in EconPapers)
Date: 2023
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