Hydrogen Production from Enzymatic Pretreated Organic Waste with Thermotoga neapolitana

Julian Tix, Fabian Moll, Simone Krafft, Matthias Betsch and Nils Tippkötter ()
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Julian Tix: Bioprocess Engineering, FH Aachen University of Applied Sciences, Campus Juelich, 52428 Juelich, Germany
Fabian Moll: Bioprocess Engineering, FH Aachen University of Applied Sciences, Campus Juelich, 52428 Juelich, Germany
Simone Krafft: Bioprocess Engineering, FH Aachen University of Applied Sciences, Campus Juelich, 52428 Juelich, Germany
Matthias Betsch: Elsa-Brandström-Straße 27, 52134 Herzogenrath, Germany
Nils Tippkötter: Bioprocess Engineering, FH Aachen University of Applied Sciences, Campus Juelich, 52428 Juelich, Germany

Energies, 2024, vol. 17, issue 12, 1-20

Abstract: Biomass from various types of organic waste was tested for possible use in hydrogen production. The composition consisted of lignified samples, green waste, and kitchen scraps such as fruit and vegetable peels and leftover food. For this purpose, the enzymatic pretreatment of organic waste with a combination of five different hydrolytic enzymes (cellulase, amylase, glucoamylase, pectinase and xylase) was investigated to determine its ability to produce hydrogen (H 2 ) with the hydrolyzate produced here. In course, the anaerobic rod-shaped bacterium T. neapolitana was used for H 2 production. First, the enzymes were investigated using different substrates in preliminary experiments. Subsequently, hydrolyses were carried out using different types of organic waste. In the hydrolysis carried out here for 48 h, an increase in glucose concentration of 481% was measured for waste loads containing starch, corresponding to a glucose concentration at the end of hydrolysis of 7.5 g·L −1 . In the subsequent set fermentation in serum bottles, a H 2 yield of 1.26 mmol H 2 was obtained in the overhead space when Terrific Broth Medium with glucose and yeast extract (TBGY medium) was used. When hydrolyzed organic waste was used, even a H 2 yield of 1.37 mmol could be achieved in the overhead space. In addition, a dedicated reactor system for the anaerobic fermentation of T. neapolitana to produce H 2 was developed. The bioreactor developed here can ferment anaerobically with a very low loss of produced gas. Here, after 24 h, a hydrogen concentration of 83% could be measured in the overhead space.

Keywords: biological hydrogen; organic waste; dark fermentation; hydrolysis; pretreatment (search for similar items in EconPapers)
JEL-codes: Q Q0 Q4 Q40 Q41 Q42 Q43 Q47 Q48 Q49 (search for similar items in EconPapers)
Date: 2024
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