Optimization of Fermentation Parameters for Enhanced Bioethanol Production by Multistress-Tolerant Saccharomycodes ludwigii APRE2 Using Undetoxified Sugarcane Bagasse Hydrolysate

Preekamol Klanrit, Sudarat Thanonkeo, Warayutt Pilap, Jirawan Apiraksakorn, Khanittha Fiala, Ratanaporn Leesing, Mamoru Yamada and Pornthap Thanonkeo ()
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Preekamol Klanrit: Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002, Thailand
Sudarat Thanonkeo: Walai Rukhavej Botanical Research Institute, Mahasarakham University, Maha Sarakham 44150, Thailand
Warayutt Pilap: Walai Rukhavej Botanical Research Institute, Mahasarakham University, Maha Sarakham 44150, Thailand
Jirawan Apiraksakorn: Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002, Thailand
Khanittha Fiala: Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002, Thailand
Ratanaporn Leesing: Department of Microbiology, Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand
Mamoru Yamada: Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, Yamaguchi 753-8515, Japan
Pornthap Thanonkeo: Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002, Thailand

Energies, 2025, vol. 18, issue 13, 1-20

Abstract: The presence of various inhibitory compounds in lignocellulosic hydrolysates poses a significant challenge for bioethanol production, requiring yeasts with exceptional multistress tolerance. This study introduces the novel application and demonstrates the robust performance of the nonconventional yeast Saccharomycodes ludwigii APRE2 for efficient bioethanol production directly from undetoxified sugarcane bagasse hydrolysate (SBH) at 37 °C. This approach critically eliminates the need for the costly detoxification pretreatments often required in industrial processes. Initial experiments confirmed S. ludwigii APRE2’s capability to ferment undetoxified SBH. To optimize fermentation efficiency, a central composite design (CCD) approach was implemented. This statistical method identified the following precise optimal parameters: sugar concentration (143.95 g/L), diammonium phosphate (4.99 g/L), pH (4.98), yeast extract (8.94 g/L), and magnesium sulfate (2.22 g/L). Under these optimized conditions, impressive results were achieved: a maximum ethanol concentration of 38.11 g/L, productivity of 1.59 g/L·h, and yield of 0.45 g/g. Notably, the ethanol productivity and theoretical yield achieved by S. ludwigii APRE2 using this inhibitor-rich, undetoxified SBH (containing acetic acid, formic acid, furfural, and 5-(hydroxymethyl)furfural) were superior to those previously reported for other ethanologenic yeasts under similar challenging conditions. This research establishes S. ludwigii APRE2 as a highly promising and industrially viable candidate for sustainable bioethanol production from lignocellulosic biomass, with its key novelty being its superior performance on undetoxified feedstocks, potentially reducing overall production costs.

Keywords: biofuel; lignocellulosic materials; multistress-tolerant yeast; nonconventional yeast; Sacharomycodes ludwigii; sugarcane bagasse (search for similar items in EconPapers)
JEL-codes: Q Q0 Q4 Q40 Q41 Q42 Q43 Q47 Q48 Q49 (search for similar items in EconPapers)
Date: 2025
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