Ozonolysis and Enzymatic Pretreatment of Fallen Norway Maple ( Acer platanoides L.) Leaves for Anaerobic Digestion

Sebastian Gosławski, Katarzyna Pielech-Przybylska, Konrad Jastrząbek and Jarosław Domański ()
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Sebastian Gosławski: Department of Environmental Biotechnology, Faculty of Biotechnology and Food Sciences, Lodz University of Technology, 90-530 Lodz, Poland
Katarzyna Pielech-Przybylska: Institute of Fermentation Technology and Microbiology, Faculty of Biotechnology and Food Sciences, Lodz University of Technology, 90-530 Lodz, Poland
Konrad Jastrząbek: Institute of Natural Products and Cosmetics, Faculty of Biotechnology and Food Science, Lodz University of Technology, 90-537 Lodz, Poland
Jarosław Domański: Department of Environmental Biotechnology, Faculty of Biotechnology and Food Sciences, Lodz University of Technology, 90-530 Lodz, Poland

Energies, 2025, vol. 18, issue 18, 1-22

Abstract: Fallen leaves of Norway maple ( Acer platanoides L.) were evaluated as a lignocellulosic substrate for anaerobic digestion (AD). The aim was to identify the pretreatment variant that maximizes methane yield. Leaves were milled and dry sieved, and two particle size classes were selected. The 1–2 cm fraction underwent chemical pretreatment by ozonation only, whereas the 0.5–1 cm fraction was first ozonated and then subjected to biological pretreatment with stepwise dosing of an enzymatic preparation. Ozonation conditions were varied with respect to particle size, ozone concentration (O 3 ), gas flow rate, and contact time. Reducing the particle size from 1–2 cm to 0.5–1 cm increased the cumulative biogas yield by 9.7% (from 64.73 to 71.01 mL g −1 VS) and the methane yield by 13.6% (from 42.01 to 47.75 mL g −1 VS) relative to the control. These findings indicate that particle size reduction combined with ozonation and staged enzymatic dosing is a promising route to improve biomethane recovery from autumn leaf feedstocks.

Keywords: leaves; ozonolysis; enzymatic treatment; particle size; anaerobic digestion (search for similar items in EconPapers)
JEL-codes: Q Q0 Q4 Q40 Q41 Q42 Q43 Q47 Q48 Q49 (search for similar items in EconPapers)
Date: 2025
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