A Novel Miniature Culture System to Screen CO 2 -Sequestering Microalgae

Wei Han, Chunying Li, Xiaoling Miao and Guangxin Yu
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Wei Han: School of Life Science & Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China
Chunying Li: School of Life Science & Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China
Xiaoling Miao: School of Life Science & Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China
Guangxin Yu: New Energy Investment Co. Ltd., Beijing 100016, China

Energies, 2012, vol. 5, issue 11, 1-18

Abstract: In this study, a novel 96-well microplate swivel system (M96SS) was built for high-throughput screening of microalgal strains for CO 2 fixation. Cell growth under different CO 2 supply conditions (0.2, 0.4, 0.8, and 1.2 g L ?1 d ?1 ), residual nitrate, and pH value of Chlorella sp. SJTU-3, Chlorella pyrenoidosa SJTU-2, and Scenedesmus obliquus SJTU-3 were examined in the M96SS and traditional flask cultures. The dynamic data showed there was a good agreement between the systems. Two critical problems in miniature culture systems (intra-well mixing and evaporation loss) were improved by sealed vertical mixing of the M96SS. A sample screen of six microalgal species ( Chlorella sp. SJTU-3, Chlorella pyrenoidosa SJTU-2, Selenastrum capricornutum , Scenedesmus obliquus SJTU-3, Chlamydomonas sajao , Dunaliella primolecta ) was carried out in flasks and the M96SS. Chlamydomonas sajao appeared to be a robust performer (highest cell density: 1.437 g L ?1 ) in anaerobic pond water with 0.8, and 1.2 g L ?1 d ?1 CO 2 . The reliability and efficiency of the M96SS were verified through a comparison of traditional flask culture, M96SS, Lukavský’s system, and a microplate shaker.

Keywords: microalgae; miniature; screening; CO 2 fixation; wastewater (search for similar items in EconPapers)
JEL-codes: Q Q0 Q4 Q40 Q41 Q42 Q43 Q47 Q48 Q49 (search for similar items in EconPapers)
Date: 2012
References: View complete reference list from CitEc
Citations: View citations in EconPapers (2)

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