Repeated-Batch Ethanol Production from Sweet Sorghum Juice by Saccharomyces cerevisiae Immobilized on Sweet Sorghum Stalks

Pongthep Ariyajaroenwong, Pattana Laopaiboon, Prasit Jaisil and Lakkana Laopaiboon
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Pongthep Ariyajaroenwong: Graduate School, Khon Kaen University, Khon Kaen 40002, Thailand
Pattana Laopaiboon: Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002, Thailand
Prasit Jaisil: Department of Plant Science and Agricultural Resources, Faculty of Agriculture, Khon Kaen University, Khon Kaen 40002, Thailand
Lakkana Laopaiboon: Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002, Thailand

Energies, 2012, vol. 5, issue 4, 1-14

Abstract: Sweet sorghum stalks were used as a low cost carrier for immobilization of Saccharomyces cerevisiae NP 01 to produce ethanol from sweet sorghum juice. The effects on ethanol production of carrier size (6 × 6 × 6 to 20 × 20 × 20 mm 3 ) and initial cell concentrations (5 × 10 7 to 2 × 10 8 cells mL ?1 ) for cell immobilization were investigated. The ethanol production medium was the juice containing 230 g L ?1 of total sugar without nutrient supplementation. The fermentations were carried out under static conditions in 500-mL air-locked Erlenmeyer flasks at 30 °C. The results showed that the optimum size of sorghum stalk pieces for repeated-batch ethanol production was 6 × 6 × 6 mm 3 , while the optimum initial cell concentration for the immobilization was 1.0 × 10 8 cells mL ?1 . The immobilized yeast under these conditions could be used for at least eight successive batches without any losses of ethanol production efficiencies. The average ethanol concentration, productivity and yield of the eight successive batches were 99.28 ± 3.53 g L ?1 , 1.36 ± 0.05 g L ?1 h ?1 and 0.47 ± 0.03 g g ?1 , respectively.

Keywords: ethanol fermentation; immobilization; repeated-batch; sweet sorghum; Saccharomyces cerevisiae (search for similar items in EconPapers)
JEL-codes: Q Q0 Q4 Q40 Q41 Q42 Q43 Q47 Q48 Q49 (search for similar items in EconPapers)
Date: 2012
References: View references in EconPapers View complete reference list from CitEc
Citations: View citations in EconPapers (6)

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