Improving Production of Bioethanol from Duckweed ( Landoltia punctata ) by Pectinase Pretreatment

Qian Chen, Yanling Jin, Guohua Zhang, Yang Fang, Yao Xiao and Hai Zhao
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Qian Chen: Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences (CAS), Chengdu 610041, China
Yanling Jin: Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences (CAS), Chengdu 610041, China
Guohua Zhang: Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences (CAS), Chengdu 610041, China
Yang Fang: Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences (CAS), Chengdu 610041, China
Yao Xiao: Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences (CAS), Chengdu 610041, China
Hai Zhao: Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences (CAS), Chengdu 610041, China

Energies, 2012, vol. 5, issue 8, 1-14

Abstract: Landoltia punctata , a widely distributed duckweed strain with the ability to accumulate starch, was used as a novel feedstock for bioethanol production by Saccharomyces cerevisiae . To improve ethanol production, pectinase pretreatment was used to release much more glucose from L. punctata mash and the pretreatment conditions (enzyme loading, temperature and pretreatment time) for the duckweed were optimized by using a surface response design. The results showed that maximum glucose yield was 218.64 ± 3.10 mg/g dry matter, which is a 142% increase compared to the untreated mash, with a pectinase dose of 26.54 pectin transeliminase unit/g mash at 45 °C for 300 min. Pectinase pretreatment apparently changed the ultrastructure of L. punctata , as evidenced by scanning electron microscopy analysis. Further fermentation experiments were performed and 30.8 ± 0.8 g/L of ethanol concentration, 90.04% of fermentation efficiency and 2.20 g/L / h of productivity rate were achieved. This is the highest ethanol concentration reported to date using duckweed as the feedstock.

Keywords: duckweed; Landoltia punctata; pectinase; ethanol fermentation; surface response design; Saccharomyces cerevisiae (search for similar items in EconPapers)
JEL-codes: Q Q0 Q4 Q40 Q41 Q42 Q43 Q47 Q48 Q49 (search for similar items in EconPapers)
Date: 2012
References: View references in EconPapers View complete reference list from CitEc
Citations: View citations in EconPapers (4)

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