Optimization of Nitrogen and Metal Ions Supplementation for Very High Gravity Bioethanol Fermentation from Sweet Sorghum Juice Using an Orthogonal Array Design

Orawan Deesuth, Pattana Laopaiboon, Prasit Jaisil and Lakkana Laopaiboon
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Orawan Deesuth: Graduate School, Khon Kaen University, Khon Kaen 40002, Thailand
Pattana Laopaiboon: Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002, Thailand
Prasit Jaisil: Department of Plant Science and Agricultural Resources, Faculty of Agriculture, Khon Kaen University, Khon Kaen 40002, Thailand
Lakkana Laopaiboon: Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002, Thailand

Energies, 2012, vol. 5, issue 9, 1-20

Abstract: Optimization of four parameters, i.e. , zinc (Zn 2+ ), magnesium (Mg 2+ ), manganese (Mn 2+ ) and yeast extract for bioethanol production from sweet sorghum juice by Saccharomyces cerevisiae NP 01 under very high gravity (VHG, 270 g·L ?1 of total sugar) conditions was performed using an L 9 (3 4 ) orthogonal array design. The fermentation was carried out at 30 °C in 500-mL air-locked Erlenmeyer flasks at the agitation rate of 100 rpm and the initial yeast cell concentration in the juice was approximately 5 × 10 7 cells·mL ?1 . The results showed that the order of influence was yeast extract > Mn 2+ > Zn 2+ > Mg 2+ and the optimum nutrient concentrations for the ethanol fermentation were Zn 2+ , 0.01; Mg 2+ , 0.05; Mn 2+ , 0.04; and yeast extract, 9 g·L ?1 . The verification experiments under the optimum condition clearly indicated that the metals and nitrogen supplementation improved ethanol production efficiency under the VHG fermentation conditions. The ethanol concentration ( P ), yield ( Y p/s ) and productivity ( Q p ) were 120.58 ± 0.26 g·L ?1 , 0.49 ± 0.01 and 2.51 ± 0.01 g·L ?1 ·h ?1 , respectively, while in the control treatment (without nutrient supplement) P , Y p/s and Q p were only 93.45 ± 0.45 g·L ?1 , 0.49 ± 0.00 and 1.30 ± 0.01 g·L ?1 ·h ?1 , respectively.

Keywords: bioethanol; trace elements; nitrogen source; orthogonal array design; Saccharomyces cerevisiae; sweet sorghum juice; very high gravity (VHG) fermentation (search for similar items in EconPapers)
JEL-codes: Q Q0 Q4 Q40 Q41 Q42 Q43 Q47 Q48 Q49 (search for similar items in EconPapers)
Date: 2012
References: View references in EconPapers View complete reference list from CitEc
Citations: View citations in EconPapers (3)

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