Microbial Community Response to Seasonal Temperature Variation in a Small-Scale Anaerobic Digester

Richard J. Ciotola, Jay F. Martin, Juan M. Castańo, Jiyoung Lee and Frederick Michel
Additional contact information
Richard J. Ciotola: Department of Food, Agricultural and Biological Engineering, The Ohio State University, Agricultural Engineering Building, 590 Woody Hayes Dr., Columbus, OH 43210-1057, USA
Jay F. Martin: Department of Food, Agricultural and Biological Engineering, The Ohio State University, Agricultural Engineering Building, 590 Woody Hayes Dr., Columbus, OH 43210-1057, USA
Juan M. Castańo: Facultad de Ciencias Ambientales, Universidad Tecnológica de Pereira (Faculty of Environmental Sciences, Technological University of Pereira), Apartado Aéreo 097, Pereira 660017, Colombia
Jiyoung Lee: Division of Environmental Health Sciences, College of Public Health, The Ohio State University, 406 Cunz Hall, 1841 Neil Avenue, Columbus, OH 43210-1057, USA
Frederick Michel: Department of Food, Agricultural and Biological Engineering, The Ohio State University, Agricultural Engineering Bldg. Rm. 107, OARDC, Wooster, OH 44691, USA

Energies, 2013, vol. 6, issue 10, 1-18

Abstract: The Bacterial and Archaeal communities in a 1.14 m 3 ambient temperature anaerobic digester treating dairy cow manure were investigated using terminal restriction fragment length polymorphisms (T-RFLP) and direct sequencing of the cloned polymerase chain reaction (PCR) products. Results indicate shifts in the structure of the both the Archaeal and Bacterial communities coincided with digester re-inoculation as well as temperature and loading rate changes. Following re-inoculation of the sour digester, the predominant Archaea shifted from Methanobrevibacter to Methanosarcina, which was the most abundant Archaea in the inoculum. Methonosarcina was replaced by Methanosaeta after the resumption of digester loading in the summer of 2010. Methanosaeta began to decline in abundance as the digester temperature cooled in the fall of 2010 while Methanobrevibacter increased in abundance. The microbial community rate of change was variable during the study period, with the most rapid changes occurring after re-inoculation.

Keywords: anaerobic digestion; fixed-dome digester; microbial communities; psychrophilic; T-RFLP (search for similar items in EconPapers)
JEL-codes: Q Q0 Q4 Q40 Q41 Q42 Q43 Q47 Q48 Q49 (search for similar items in EconPapers)
Date: 2013
References: View references in EconPapers View complete reference list from CitEc
Citations: View citations in EconPapers (4)

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