Measurement of Lipid Droplet Accumulation Kinetics in Chlamydomonas reinhardtii Using Seoul-Fluor

Jae Woo Park, Sang Cheol Na, Youngjun Lee, Sanghee Lee, Seung Bum Park and Noo Li Jeon
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Jae Woo Park: Division of WCU (World Class University) Multiscale Mechanical Design, School of Mechanical Engineering, Seoul National University, Seoul 151-744, Korea
Sang Cheol Na: Division of WCU (World Class University) Multiscale Mechanical Design, School of Mechanical Engineering, Seoul National University, Seoul 151-744, Korea
Youngjun Lee: Department of Chemistry, Seoul National University, Seoul 151-744, Korea
Sanghee Lee: Department of Chemistry, Seoul National University, Seoul 151-744, Korea
Seung Bum Park: Department of Chemistry, Seoul National University, Seoul 151-744, Korea
Noo Li Jeon: Division of WCU (World Class University) Multiscale Mechanical Design, School of Mechanical Engineering, Seoul National University, Seoul 151-744, Korea

Energies, 2013, vol. 6, issue 11, 1-14

Abstract: Alternative energy resources have become an important issue due to the limited stocks of petroleum-based fuel. Microalgae, a source of renewable biodiesel, use solar light to convert CO 2 into lipid droplets (LDs). Quantification of LDs in microalgae is required for developing and optimizing algal bioprocess engineering. However, conventional quantification methods are both time and labor-intensive and difficult to apply in high-throughput screening systems. LDs in plant and mammalian cells can be visualized by staining with various fluorescence probes such as the Nile Red, BODIPY, and Seoul-Fluor (SF) series. This report describes the optimization of LD staining in Chlamydomonas reinhardtii with SF probes via systematic variations of dye concentration, staining time, temperature, and pH. A protocol for quantitative measurement of accumulation kinetics of LDs in C. reinhardtii was developed using a spectrofluorimeter and the accuracy of LD size measurement was confirmed by transmission electron microscopy (TEM). Our results indicate that our spectrofluorimeter-based measurement approach can monitor kinetics of intracellular LDs (in control and nitrogen-source-starved Chlamydomonas reinhardtii ) accumulation that has not been possible in the case of conventional imaging-based methods. Our results presented here confirmed that an SF44 can be a powerful tool for in situ monitoring and tracking of intracellular LDs formation.

Keywords: microalgae; Chlamydomonas reinhardtii; SF44; lipid droplets; biodiesel (search for similar items in EconPapers)
JEL-codes: Q Q0 Q4 Q40 Q41 Q42 Q43 Q47 Q48 Q49 (search for similar items in EconPapers)
Date: 2013
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