Optimizing the Production of Biodiesel Using Lipase Entrapped in Biomimetic Silica

I-Ching Kuan, Chia-Chi Lee, Bing-Hong Tsai, Shiow-Ling Lee, Wei-Ting Lee and Chi-Yang Yu
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I-Ching Kuan: Department of Bioengineering, Tatung University, 40 Zhongshan N. Rd. Sec. 3, Taipei 10452, Taiwan
Chia-Chi Lee: Department of Bioengineering, Tatung University, 40 Zhongshan N. Rd. Sec. 3, Taipei 10452, Taiwan
Bing-Hong Tsai: Department of Bioengineering, Tatung University, 40 Zhongshan N. Rd. Sec. 3, Taipei 10452, Taiwan
Shiow-Ling Lee: Department of Bioengineering, Tatung University, 40 Zhongshan N. Rd. Sec. 3, Taipei 10452, Taiwan
Wei-Ting Lee: Department of Bioengineering, Tatung University, 40 Zhongshan N. Rd. Sec. 3, Taipei 10452, Taiwan
Chi-Yang Yu: Department of Bioengineering, Tatung University, 40 Zhongshan N. Rd. Sec. 3, Taipei 10452, Taiwan

Energies, 2013, vol. 6, issue 4, 1-13

Abstract: We entrapped lipase from Pseudomonas cepacia in polyallylamine-mediated biomimetic silica, and then applied entrapped lipase to the synthesis of biodiesel with soybean oil or waste cooking oil as a feedstock. The effects of reaction temperature, substrate molar ratio (methanol/oil) and n -hexane content (w/w of oil) were evaluated using response surface methodology (RSM) combined with Box-Behnken design. The optimal reaction conditions for soybean oil were 43.6 °C, substrate molar ratio of 4.3%, and 75% n -hexane. The predicted and experimental values of biodiesel conversion were 79% and 76%, respectively. The optimal reaction conditions for waste cooking oil were 43.3 °C, substrate molar ratio of 5%, and 38% n -hexane. The predicted and experimental values of conversion were 68% and 67%, respectively. The conversion efficiency remained the same even after 1-month storage of entrapped lipase at 4 °C or room temperature.

Keywords: biodiesel; lipase; biomimetic silica; response surface methodology (search for similar items in EconPapers)
JEL-codes: Q Q0 Q4 Q40 Q41 Q42 Q43 Q47 Q48 Q49 (search for similar items in EconPapers)
Date: 2013
References: View references in EconPapers View complete reference list from CitEc
Citations: View citations in EconPapers (2)

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