Batch Growth of Chlorella Vulgaris CCALA 896 versus Semi-Continuous Regimen for Enhancing Oil-Rich Biomass Productivity

Sigita Vaičiulytė, Giulia Padovani, Jolanta Kostkevičienė and Pietro Carlozzi
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Sigita Vaičiulytė: Department of Botany and Genetics, Faculty of Natural Sciences, Vilnius University, M.K.Ciurlionio 21/27, Vilnius LT-03101, Lithuania
Giulia Padovani: Institute of Ecosystem Study, National Research Council (CNR), Section of Florence, Polo Scientifico, Via Madonna del Piano n. 10, Sesto Fiorentino (FI) IT-50019, Italy
Jolanta Kostkevičienė: Department of Botany and Genetics, Faculty of Natural Sciences, Vilnius University, M.K.Ciurlionio 21/27, Vilnius LT-03101, Lithuania
Pietro Carlozzi: Institute of Ecosystem Study, National Research Council (CNR), Section of Florence, Polo Scientifico, Via Madonna del Piano n. 10, Sesto Fiorentino (FI) IT-50019, Italy

Energies, 2014, vol. 7, issue 6, 1-18

Abstract: The aim of this study was to induce lipid accumulation in Chlorella cells by creating stressful growth conditions. Chlorella vulgaris CCALA 896 was grown under various batch growth modes in basal and modified BG-11 and Kolkwitz culture broths, using a continuous light regimen of 150 µE/m 2 /s, at 30 °C. In order to perform the experiments, two indoor photobioreactor shapes were used: a cylindrical glass photobioreactor (C G PBR) with a working volume of 350 mL, and a flat glass photobioreactor (F G PBR) with a working volume of 550 mL. Stress-eliciting conditions, such as nitrogen and phosphorous starvation, were imposed in order to induce lipid accumulation. The results demonstrated that more than 56% of the lipids can be accumulated in Chlorella biomass grown under two-phase batch growth conditions. The highest biomass productivity of 0.30 g/L/d was obtained at the highest nominal dilution rate (0.167 day ?1 ) during a semi-continuous regimen, using a modified Kolkwitz medium. During the pH-stress cycles, the amount of lipids did not increase significantly and a flocculation of Chlorella cells was noted.

Keywords: Chlorella vulgaris; indoor photobioreactors; nitrogen/phosphorus starvation; biomass productivity; oil-rich biomass (search for similar items in EconPapers)
JEL-codes: Q Q0 Q4 Q40 Q41 Q42 Q43 Q47 Q48 Q49 (search for similar items in EconPapers)
Date: 2014
References: View references in EconPapers View complete reference list from CitEc
Citations: View citations in EconPapers (4)

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