Evaluation of Cell Disruption of Chlorella Vulgaris by Pressure-Assisted Ozonation and Ultrasonication

Yuanxing Huang, Shengnan Qin, Daofang Zhang, Liang Li and Yan Mu
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Yuanxing Huang: School of Environment and Architecture, University of Shanghai for Science and Technology, Shanghai 200093, China
Shengnan Qin: School of Environment and Architecture, University of Shanghai for Science and Technology, Shanghai 200093, China
Daofang Zhang: School of Environment and Architecture, University of Shanghai for Science and Technology, Shanghai 200093, China
Liang Li: School of Environment and Architecture, University of Shanghai for Science and Technology, Shanghai 200093, China
Yan Mu: Hebei Province Environmental Monitoring Center, Shijiazhuang 050056, China

Energies, 2016, vol. 9, issue 3, 1-11

Abstract: This study evaluated the effectiveness of pressure-assisted ozonation (PAO) in Chlorella vulgaris ( C. vulgaris ) cell disruption, and compared the disruption result with that of the ultrasonication (US) by using four quantification indicators: cell counting, ultra violet (UV) absorbance, turbidity and visible light absorbance. It was found that under the condition of 0.8 MPa and 80 cycles, PAO treatment achieved cell rupture of 80.3%, with the power of 1080 W and treatment time of 60 min, US achieved cell rupture of 83.8%. Cell counting was a reliable indicator and applicable to both PAO and US treatments. Turbidity and visible light absorbance gave similar results and featured as the simplest operation. UV absorbance reflected the metabolite release due to cell breakage; however, it was less reproducible when it was applied to quantify the cell rupture by PAO. Its trend indicated that during cell disruption metabolite degradation occurred, especially after significant rupture in the case of excessive PAO treatment. The cellular morphology of C. vulgaris cells during PAO and US treatments was investigated by scanning electron microscope (SEM) which certified that the cells damage was caused by both physical and chemical attack.

Keywords: cell disrupture; quantitative evaluation; Chlorella vulgaris ( C. vulgaris ); pressure-assisted ozonation (PAO); ultrasonication (US) (search for similar items in EconPapers)
JEL-codes: Q Q0 Q4 Q40 Q41 Q42 Q43 Q47 Q48 Q49 (search for similar items in EconPapers)
Date: 2016
References: View references in EconPapers View complete reference list from CitEc
Citations: View citations in EconPapers (1)

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