Genetic Detection of Pseudomonas spp. in Commercial Amazonian Fish

Alba Ardura, Ana R. Linde and Eva Garcia-Vazquez
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Alba Ardura: Department of Functional Biology, University of Oviedo. C/Julian Claveria s/n, Oviedo 33006, Spain
Ana R. Linde: Laboratory of Toxicology, National School of Public Health, Fundaçao Oswaldo Cruz, Rio de Janeiro 21041, Brazil
Eva Garcia-Vazquez: Department of Functional Biology, University of Oviedo. C/Julian Claveria s/n, Oviedo 33006, Spain

IJERPH, 2013, vol. 10, issue 9, 1-13

Abstract: Brazilian freshwater fish caught from large drainages like the River Amazon represent a million ton market in expansion, which is of enormous importance for export to other continents as exotic seafood. A guarantee of bacteriological safety is required for international exports that comprise a set of different bacteria but not any Pseudomonas . However, diarrhoea, infections and even septicaemia caused by some Pseudomonas species have been reported, especially in immune-depressed patients. In this work we have employed PCR-based methodology for identifying Pseudomonas species in commercial fish caught from two different areas within the Amazon basin. Most fish caught from the downstream tributary River Tapajòs were contaminated by five different Pseudomonas species. All fish samples obtained from the River Negro tributary (Manaus markets) contained Pseudomonas , but a less diverse community with only two species. The most dangerous Pseudomonas species for human health, P. aeruginosa , was not found and consumption of these fish (from their Pseudomonas content) can be considered safe for healthy consumers. As a precautionary approach we suggest considering Pseudomonas in routine bacteriological surveys of imported seafood.

Keywords: Amazon River; Pseudomonas; molecular tests; commercial fish; food safety (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2013
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