Genotypes and Antibiotic Resistances of Campylobacter jejuni Isolates from Cattle and Pigeons in Dairy Farms

Valentina Bianchini, Mario Luini, Laura Borella, Antonio Parisi, Romie Jonas, Sonja Kittl and Peter Kuhnert
Additional contact information
Valentina Bianchini: Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna, Lodi 26900, Italy
Mario Luini: Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna, Lodi 26900, Italy
Laura Borella: Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna, Lodi 26900, Italy
Antonio Parisi: Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata, Putignano (BA) 70017, Italy
Romie Jonas: Institute of Veterinary Bacteriology, Vetsuisse Faculty, University of Bern, Bern CH-3001, Switzerland
Sonja Kittl: Institute of Veterinary Bacteriology, Vetsuisse Faculty, University of Bern, Bern CH-3001, Switzerland
Peter Kuhnert: Institute of Veterinary Bacteriology, Vetsuisse Faculty, University of Bern, Bern CH-3001, Switzerland

IJERPH, 2014, vol. 11, issue 7, 1-9

Abstract: Campylobacter jejuni is the most common food-borne zoonotic pathogen causing human gastroenteritis worldwide and has assumed more importance in Italy following the increased consumption of raw milk. Our objectives were to get an overview of genotypes and antibiotic resistances in C. jejuni isolated from milk, cattle feces, and pigeons in dairy herds of Northern Italy. flaB -typing was applied to 78 C. jejuni isolates, previously characterized by Multi-Locus Sequence Typing, and genotypic resistances towards macrolides and quinolones based on point mutations in the 23S rRNA and gyrA genes, respectively, were determined. flaB -typing revealed 22 different types with one of them being novel and was useful to further differentiate strains with an identical Sequence Type (ST) and to identify a pigeon-specific clone. Macrolide resistance was not found, while quinolone resistance was detected in 23.3% of isolates. A relationship between specific genotypes and antibiotic resistance was observed, but was only significant for the Clonal Complex 206. Our data confirm that pigeons do not play a role in the spread of C. jejuni among cattle and they are not responsible for milk contamination. A relevant number of bulk milk samples were contaminated by C. jejuni resistant to quinolones, representing a possible source of human resistant strains.

Keywords: Campylobacter jejuni; dairy farms; pigeons; genotyping; antibiotic resistance (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2014
References: View complete reference list from CitEc
Citations:

Downloads: (external link)
https://www.mdpi.com/1660-4601/11/7/7154/pdf (application/pdf)
https://www.mdpi.com/1660-4601/11/7/7154/ (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:gam:jijerp:v:11:y:2014:i:7:p:7154-7162:d:38094

Access Statistics for this article

IJERPH is currently edited by Ms. Jenna Liu

More articles in IJERPH from MDPI
Bibliographic data for series maintained by MDPI Indexing Manager ().