Cytotoxic Evaluation of e-Liquid Aerosol using Different Lung-Derived Cell Models

Stefanie Scheffler, Hauke Dieken, Olaf Krischenowski and Michaela Aufderheide
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Stefanie Scheffler: Cultex Laboratories GmbH, Feodor-Lynen-Street 21, 30625 Hannover, Germany
Hauke Dieken: Cultex Laboratories GmbH, Feodor-Lynen-Street 21, 30625 Hannover, Germany
Olaf Krischenowski: Cultex Laboratories GmbH, Feodor-Lynen-Street 21, 30625 Hannover, Germany
Michaela Aufderheide: Cultex Laboratories GmbH, Feodor-Lynen-Street 21, 30625 Hannover, Germany

IJERPH, 2015, vol. 12, issue 10, 1-9

Abstract: The in vitro toxicological evaluation of e-liquid aerosol is an important aspect of consumer protection, but the cell model is of great significance. Due to its water solubility, e-liquid aerosol is deposited in the conducting zone of the respiratory tract. Therefore, primary normal human bronchial epithelial (NHBE) cells are more suitable for e-liquid aerosol testing than the widely used alveolar cell line A549. Due to their prolonged lifespan, immortalized cell lines derived from primary NHBE cells, exhibiting a comparable in vitro differentiation, might be an alternative for acute toxicity testing. In our study, A549 cells freshly isolated NHBE cells and the immortalized cell line CL-1548 were exposed at the air-liquid interface to e-liquid aerosol and cigarette mainstream smoke in a CULTEX ® RFS compact module. The cell viability was analyzed 24 h post-exposure. In comparison with primary NHBE cells, the CL-1548 cell line showed lower sensitivity to e-liquid aerosol but significantly higher sensitivity compared to A549 cells. Therefore, the immortalized cell line CL-1548 is recommended as a tool for the routine testing of e-liquid aerosol and is preferable to A549 cells.

Keywords: electronic cigarette; smoking; tobacco; nicotine; cytotoxicity; immortalized normal bronchial epithelial cells; air-liquid interface; CL-1548; CULTEX ® RFS; public health (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2015
References: View references in EconPapers View complete reference list from CitEc
Citations: View citations in EconPapers (2)

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