Thyroid Disruption in Zebrafish Larvae by Short-Term Exposure to Bisphenol AF

Tianle Tang, Yang Yang, Yawen Chen, Wenhao Tang, Fuqiang Wang and Xiaoping Diao
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Tianle Tang: College of Environment and Plant Protection, Hainan University, Haikou 570228, China
Yang Yang: College of Environment and Plant Protection, Hainan University, Haikou 570228, China
Yawen Chen: College of Environment and Plant Protection, Hainan University, Haikou 570228, China
Wenhao Tang: College of Environment and Plant Protection, Hainan University, Haikou 570228, China
Fuqiang Wang: College of Environment and Plant Protection, Hainan University, Haikou 570228, China
Xiaoping Diao: Haikou Key Laboratory of Environment Toxicology, Hainan University, Haikou 570228, China

IJERPH, 2015, vol. 12, issue 10, 1-16

Abstract: Bisphenol AF (BPAF) is extensively used as a raw material in industry, resulting in its widespread distribution in the aqueous environment. However, the effect of BPAF on the hypothalamic-pituitary-thyroidal (HPT) axis remains unknown. For elucidating the disruptive effects of BPAF on thyroid function and expression of the representative genes along the HPT axis in zebrafish ( Danio rerio ) embryos, whole-body total 3,3?,5-triiodothyronine (TT3), total 3,5,3?,5?-tetraiodothyronine (TT4), free 3,3?,5-triiodothyronine (FT3) and free 3,5,3?,5?-tetraiodothyronine (FT4) levels were examined following 168 h post-fertilization exposure to different BPAF concentrations (0, 5, 50 and 500 ?g/L). The results showed that whole-body TT3, TT4, FT3 and FT4 contents decreased significantly with the BPAF treatment, indicating an endocrine disruption of thyroid. The expression of thyroid-stimulating hormone-? and thyroglobulin genes increased after exposing to 50 ?g/L BPAF in seven-day-old larvae. The expressions of thyronine deiodinases type 1, type 2 and transthyretin mRNAs were also significantly up-regulated, which were possibly associated with a deterioration of thyroid function. However, slc5a5 gene transcription was significantly down-regulated at 50 ?g/L and 500 ?g/L BPAF exposure. Furthermore, tr ? and tr ? genes were down-regulated transcriptionally after BPAF exposure. It demonstrates that BPAF exposure triggered thyroid endocrine toxicity by altering the whole-body contents of thyroid hormones and changing the transcription of the genes involved in the HPT axis in zebrafish larvae.

Keywords: bisphenol AF (BPAF); zebrafish ( Danio rerio ); thyroid hormones (THs); hypothalamic-pituitary-thyroid (HPT) axis (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2015
References: View complete reference list from CitEc
Citations: View citations in EconPapers (2)

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