Commercial Honeybush ( Cyclopia spp. ) Tea Extract Inhibits Osteoclast Formation and Bone Resorption in RAW264.7 Murine Macrophages—An in vitro Study

Amcois Visagie, Abe Kasonga, Vishwa Deepak, Shaakirah Moosa, Sumari Marais, Marlena C. Kruger and Magdalena Coetzee
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Amcois Visagie: Department of Physiology, Faculty of Health Sciences, University of Pretoria, Pretoria 0001, South Africa
Abe Kasonga: Department of Physiology, Faculty of Health Sciences, University of Pretoria, Pretoria 0001, South Africa
Vishwa Deepak: Department of Physiology, Faculty of Health Sciences, University of Pretoria, Pretoria 0001, South Africa
Shaakirah Moosa: Department of Physiology, Faculty of Health Sciences, University of Pretoria, Pretoria 0001, South Africa
Sumari Marais: Department of Physiology, Faculty of Health Sciences, University of Pretoria, Pretoria 0001, South Africa
Marlena C. Kruger: School of Food and Nutrition, Massey Institute for Food Science and Technology, Massey University, Palmerston North 4442, New Zealand
Magdalena Coetzee: Department of Physiology, Faculty of Health Sciences, University of Pretoria, Pretoria 0001, South Africa

IJERPH, 2015, vol. 12, issue 11, 1-15

Abstract: Honeybush tea, a sweet tasting caffeine-free tea that is indigenous to South Africa, is rich in bioactive compounds that may have beneficial health effects. Bone remodeling is a physiological process that involves the synthesis of bone matrix by osteoblasts and resorption of bone by osteoclasts. When resorption exceeds formation, bone remodeling can be disrupted resulting in bone diseases such as osteoporosis. Osteoclasts are multinucleated cells derived from hematopoietic precursors of monocytic lineage. These precursors fuse and differentiate into mature osteoclasts in the presence of receptor activator of NF-kB ligand (RANKL), produced by osteoblasts. In this study, the in vitro effects of an aqueous extract of fermented honeybush tea were examined on osteoclast formation and bone resorption in RAW264.7 murine macrophages. We found that commercial honeybush tea extract inhibited osteoclast formation and TRAP activity which was accompanied by reduced bone resorption and disruption of characteristic cytoskeletal elements of mature osteoclasts without cytotoxicity. Furthermore, honeybush tea extract decreased expression of key osteoclast specific genes, matrix metalloproteinase-9 (MMP-9), tartrate resistant acid phosphatase (TRAP) and cathepsin K. This study demonstrates for the first time that honeybush tea may have potential anti-osteoclastogenic effects and therefore should be further explored for its beneficial effects on bone.

Keywords: honeybush tea; osteoclast; bone resorption; RANKL; RAW264.7 murine macrophages (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2015
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