Investigation of the Effects of Perfluorooctanoic Acid (PFOA) and Perfluorooctane Sulfonate (PFOS) on Apoptosis and Cell Cycle in a Zebrafish ( Danio rerio ) Liver Cell Line

Yuan Cui, Wei Liu, Wenping Xie, Wenlian Yu, Cheng Wang and Huiming Chen
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Yuan Cui: Chinese Academy of Inspection and Quarantine, Beijing 100123, China
Wei Liu: Chinese Academy of Inspection and Quarantine, Beijing 100123, China
Wenping Xie: Chinese Academy of Inspection and Quarantine, Beijing 100123, China
Wenlian Yu: Chinese Academy of Inspection and Quarantine, Beijing 100123, China
Cheng Wang: Chinese Academy of Inspection and Quarantine, Beijing 100123, China
Huiming Chen: Chinese Academy of Inspection and Quarantine, Beijing 100123, China

IJERPH, 2015, vol. 12, issue 12, 1-10

Abstract: This study aimed to explore the effects of perfluorooctanoic acid (PFOA) and perfluorooctane sulfonate (PFOS) on apoptosis and cell cycle in a zebrafish ( Danio rerio ) liver cell line (ZFL). Treatment groups included a control group, PFOA-IC 50 , PFOA-IC 80 , PFOS-IC 50 and PFOS-IC 80 groups. IC 50 and IC 80 concentrations were identified by cellular modeling and MTT assays. mRNA levels of p53, Bcl-2, Bax, Caspase-3 and NF-?B p65 were detected by qPCR. Cell apoptosis and cell cycle were detected by flow cytometry and the protein levels of p53, Bcl-2, Bax, Caspase-3 and NF-?B p65 were determined by western blotting. Both PFOA and PFOS inhibited the growth of zebrafish liver cells, and the inhibition rate of PFOS was higher than that of PFOA. Bcl-2 expression levels in the four groups were significantly higher than the control group and Bcl-2 increased significantly in the PFOA-IC 80 group. However, the expression levels of Bax in the four treatment groups were higher than the control group. The percentage of cell apoptosis increased significantly with the treatment of PFOA and PFOS ( p < 0.05). Cell cycle and cell proliferation were blocked in both the PFOA-IC 80 and PFOS-IC 80 groups, indicating that PFOA-IC 80 and PFOS-IC 50 enhanced apoptosis in ZFL cells.

Keywords: perfluorooctanoic acid (PFOA); perfluorooctane sulfonate (PFOS); zebrafish liver cells (ZFL); apoptosis (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2015
References: View complete reference list from CitEc
Citations: View citations in EconPapers (1)

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