Production of the Allergenic Protein Alt a 1 by Alternaria Isolates from Working Environments

Justyna Skóra, Anna Otlewska, Beata Gutarowska, Joanna Leszczyńska, Iwona Majak and Łukasz Stępień
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Justyna Skóra: Institute of Fermentation Technology and Microbiology, Lodz University of Technology, 171/173 Wólczańska Str, 90-924 Łódź, Poland
Anna Otlewska: Institute of Fermentation Technology and Microbiology, Lodz University of Technology, 171/173 Wólczańska Str, 90-924 Łódź, Poland
Beata Gutarowska: Institute of Fermentation Technology and Microbiology, Lodz University of Technology, 171/173 Wólczańska Str, 90-924 Łódź, Poland
Joanna Leszczyńska: Institute of General Food Chemistry, Lodz University of Technology, 171/173 Wólczańska Str, 90-924 Łódź, Poland
Iwona Majak: Institute of General Food Chemistry, Lodz University of Technology, 171/173 Wólczańska Str, 90-924 Łódź, Poland
Łukasz Stępień: Institute of Plant Genetics, Polish Academy of Sciences, 34 Strzeszyńska Str, 60-479 Poznań, Poland

IJERPH, 2015, vol. 12, issue 2, 1-20

Abstract: The aim of the study was to evaluate the ability of Alternaria isolates from workplaces to produce Alt a 1 allergenic protein, and to analyze whether technical materials (cellulose, compost, leather) present within the working environment stimulate or inhibit Alt a 1 production (ELISA test). Studies included identification of the isolated molds by nucleotide sequences analyzing of the ITS1/ITS2 regions, actin, calmodulin and Alt a 1 genes. It has been shown that Alternaria molds are significant part of microbiocenosis in the archive, museum, library, composting plant and tannery (14%–16% frequency in the air). The presence of the gene encoding the Alt a 1 protein has been detected for the strains: Alternaria alternata , A. lini , A. limoniasperae A. nobilis and A. tenuissima. Environmental strains produced Alt a 1 at higher concentrations (1.103–6.528 ng/mL) than a ATCC strain (0.551–0.975 ng/mL). It has been shown that the homogenization of the mycelium and the use of ultrafiltration allow a considerable increase of Alt a 1 concentration. Variations in the production of Alt a 1 protein, depend on the strain and extraction methods. These studies revealed no impact of the technical material from the workplaces on the production of Alt a 1 protein.

Keywords: Alternaria; workplaces; Alt a 1 protein; Alt a 1 gene; ELISA (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2015
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