Method Comparison for Enhanced Recovery, Isolation and Qualitative Detection of C. jejuni and C. coli from Wastewater Effluent Samples

María Ugarte-Ruiz, Diego Florez-Cuadrado, Trudy M. Wassenaar, María Concepción Porrero and Lucas Domínguez
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María Ugarte-Ruiz: VISAVET Health Surveillance Centre, Complutense University of Madrid, Avda. Puerta de Hierro, s/n. 28040 Madrid, Spain
Diego Florez-Cuadrado: VISAVET Health Surveillance Centre, Complutense University of Madrid, Avda. Puerta de Hierro, s/n. 28040 Madrid, Spain
Trudy M. Wassenaar: Molecular Microbiology and Genomics Consultants, Zotzenheim 55576, Germany
María Concepción Porrero: VISAVET Health Surveillance Centre, Complutense University of Madrid, Avda. Puerta de Hierro, s/n. 28040 Madrid, Spain
Lucas Domínguez: VISAVET Health Surveillance Centre, Complutense University of Madrid, Avda. Puerta de Hierro, s/n. 28040 Madrid, Spain

IJERPH, 2015, vol. 12, issue 3, 1-16

Abstract: Seeking a sensitive protocol, culture-dependent methods were compared to detect thermophilic Campylobacter species in untreated urban effluents. We evaluated various combinations of selective media, with and without an enrichment steps, as well as an extra filtration step. Culture-independent real-time quantitative PCR was also included and all detected isolates underwent antimicrobial susceptibility testing. All tested water samples contained Campylobacter DNA, but only 64% were positive after culture. Although enrichment using Preston broth resulted in better recovery of potentially stressed Campylobacter than Bolton or Campyfood broth (CFB), there was no significant increase in efficiency compared to direct plating. The type of selective agar media used, on the other hand, had a significant effect, with CASA plates performing better than mCCDA or CFA ones. Inclusion of an enrichment step increased the ratio of C. coli vs. C. jejuni being isolated. Resistances against all antimicrobials tested were observed in C. coli , but fewer instances of resistance were found in C. jejuni isolates. Whether this difference was the result of selection during the enrichment step could not be determined. The presence of Campylobacter in urban effluents can be considered as a valuable proxy for Campylobacter populations present in urban environments.

Keywords: Campylobacter; urban effluents; isolation protocols; antimicrobial resistance (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2015
References: View references in EconPapers View complete reference list from CitEc
Citations: View citations in EconPapers (2)

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