Diuron in Water: Functional Toxicity and Intracellular Detoxification Patterns of Active Concentrations Assayed in Tandem by a Yeast-Based Probe

Roberto Dragone, Rachel Cheng, Gerardo Grasso and Chiara Frazzoli
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Roberto Dragone: Institute for Nanostructured Materials (ISMN), National Research Council, 00185 Rome, Italy
Rachel Cheng: Institute for Nanostructured Materials (ISMN), National Research Council, 00185 Rome, Italy
Gerardo Grasso: Institute for Nanostructured Materials (ISMN), National Research Council, 00185 Rome, Italy
Chiara Frazzoli: External Relations Office, National Institute of Health, 00162 Rome, Italy

IJERPH, 2015, vol. 12, issue 4, 1-10

Abstract: A study on the acute and chronic effects of the herbicide diuron was carried out. The test, basing on a yeast cell probe, investigated the interference with cellular catabolism and possible self-detoxification capacity of Saccharomyces cerevisiae . Aerobic respiration was taken as the toxicological end-point. Percentage interference (%r) with cellular respiration was measured in water by increased dissolved O 2 concentration (ppm) after exposure to different doses. Interference was calculated through the comparison of respiratory activity of exposed and non-exposed cells. Short-term and long-term (6 and 24 h respectively) exposures were also considered. The test for short-term exposure gave positive %r values except that for 10 ?6 M (11.11%, 11.76%, 13.33% and 0% for 10 ?10 M, 10 ?8 M, 10 ?7 M and 10 ?6 M respectively). In the case of long-term exposure the test showed positive %r values, but less effect than short-term exposure until 10 ?8 M and much higher at 10 ?6 M (7.41%, 8.82%, 11.76% and 6.06% for 10 ?10 M, 10 ?8 M, 10 ?7 M and 10 ?6 M respectively). The findings of aerobic respiration as toxicological end-point were in agreement with known mechanisms of toxicity and intracellular detoxification for both the doses and exposure times employed.

Keywords: herbicide; respirometric biosensor; mitochondrial toxicity (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2015
References: View complete reference list from CitEc
Citations: View citations in EconPapers (1)

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