Development of Primer Sets for Loop-Mediated Isothermal Amplification that Enables Rapid and Specific Detection of Streptococcus dysgalactiae, Streptococcus uberis and Streptococcus agalactiae

Deguo Wang and Yanhong Liu
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Deguo Wang: Henan Postdoctoral Research Base, Food and Bioengineering College, Xuchang University, Xuchang 461000, China
Yanhong Liu: Molecular Characterization of Foodborne Pathogens Research Unit, Eastern Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture, 600 East Mermaid Lane, Wyndmoor, PA 19038, USA

IJERPH, 2015, vol. 12, issue 6, 1-8

Abstract: Streptococcus dysgalactiae , Streptococcus uberis and Streptococcus agalactiae are the three main pathogens causing bovine mastitis, with great losses to the dairy industry. Rapid and specific loop-mediated isothermal amplification methods (LAMP) for identification and differentiation of these three pathogens are not available. With the 16S rRNA gene and 16S-23S rRNA intergenic spacers as targets, four sets of LAMP primers were designed for identification and differentiation of S. dysgalactiae , S. uberis and S. agalactiae . The detection limit of all four LAMP primer sets were 0.1 pg DNA template per reaction, the LAMP method with 16S rRNA gene and 16S-23S rRNA intergenic spacers as the targets can differentiate the three pathogens, which is potentially useful in epidemiological studies.

Keywords: loop-mediated isothermal amplification (LAMP); 16S rRNA gene; 16S-23S rRNA intergenic spacer; Streptococcus dysgalactiae; Streptococcus uberis; Streptococcus agalactiae (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2015
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